CD73 Inhibitor Screening Assay Kit

Intended Use

The CD73 Inhibitor Screening Assay Kit is designed to measure CD73 activity for screening and profiling applications. The CD73 assay kit comes in a convenient 96-well format, with purified recombinant CD73 enzyme, AMP, CD73 assay buffer, and Colorimetric detection reagent for 100 enzyme reactions.

Contents of Kit

1. CD73: 5 μg, -80°C;
2. 5x CD73 assay buffer: 2 x 1 ml, -20°C;
3. AMP (500 μM): 1 ml, -20°C;
4. Colorimetric detection reagent*: 10 ml, Room Temp;
5. Transparent 96-well plate: 1, Room Temp.
*Colorimetric detection reagent is used to measure the free phosphate from the CD73 reaction. Any source of inorganic phosphate can interfere with the assay.

Storage

Up to 6 months from date of receipt, when stored as recommended.

General Description

CD73, also known as Ecto-5'-nucleotidase (5'-NT), promotes tumor growth and progression by degrading AMP into adenosine. CD73-generated adenosine blocks T-cell immuno-surveillance, resulting in an immunosuppressed and pro-angiogenic niche within the tumor microenvironment.