ApoE4/Pan-ApoE ELISA Kit

Name: ApoE4/Pan-ApoE ELISA Kit
SKU: DEIABL516
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Cell culture supernatant, Serum, Plasma, Cell Lysate, Cerebral Spinal Fluid

Species Reactivity

Human

Detection Method

sELISA

Intended Use

Quantitative measurement of human Apolipoprotein E4 (ApoE4)

Contents of Kit

1. 100× Detection Antibody, 1×110μl
2. 100× HRP-Conjugate, 1×135μl
3. 10× Wash buffer A, 1×10ml
4. 10× Wash buffer B, 1×20ml
5. ApoE A pre-coated Microplate, 1 unit
6. Assay Diluent, 1×100ml
7. Plate Sealer, 2 units
8. Rh-ApoE4 Standard (100μg/ml), 3×8μl
9. Stop Solution, 1×11ml
10. TMB Substrate, 1×11ml

Storage

Kit can be used within one year if stored unopened at -20°C. Avoid repeated freeze-thaw cycles. After opening the kit, store components according to the Storage temperature listed above. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.

Performance Characteristics

Measurement of ApoE4 concentration in human plasma samples from Alzheimer's disease (AD) patients following the kit protocol.
E3/E3 (left), two copies of ApoE3 in AD patient plasma; E4/E4 (right), two copies of ApoE4 in AD patient plasma.

Detection of ApoE4 in ApoE overexpressed HEK293T cell line. Cell lysate was spiked with rh ApoE2 or rh ApoE4 and analyzed following the kit protocol.

Detection Range

50 ng/ml-800 ng/ml

Sensitivity

25 ng/ml

General Description

ApoE transports lipoproteins, fat-soluble vitamins, and cholesterol via the lymph system to the blood. ApoE exists as three major isoforms, including ApoE2, ApoE3, and ApoE4. Recently, ApoE4 has been implicated in Alzheimer's disease (AD). ApoE4 is the first risk gene identified in Alzheimer's research, and remains the gene with strongest impact. Everyone inherits a copy of ApoE gene from each parent. Those who inherit one copy of ApoE4 have an increased risk of developing AD (about a quarter of the human population). Those who inherit two copies of ApoE4 have an even higher risk (about 2% of humans with up to 10 times higher risk). In addition to raising risk, ApoE4 may tend to make AD symptoms appear at a younger age.

Standard Curve