Analysis of the Antibiotic Resistance Profiles in Methicillin-Sensitive S. aureus Pathotypes Isolated on a Commercial Rabbit Farm in Italy
ANTIBIOTICS-BASEL
Authors: Attili, Anna-Rita; Bellato, Alessandro; Robino, Patrizia; Galosi, Livio; Papeschi, Cristiano; Rossi, Giacomo; Fileni, Eleonora; Linardi, Martina; Cuteri, Vincenzo; Chiesa, Francesco; Nebbia, Patrizia
Abstract
The breeding of meat rabbits is an important sector in the livestock industry in Italy. The focus of this study was to describe the antibiotic resistance profile distribution among the Methicillin-sensitive Staphylococcus aureus isolated in a rabbit farm. From 400 animals of different ages and three farm workers, 96 randomly selected strains isolated from various anatomical sites and lesions were analysed. According to spa typing and the resistance profiles towards veterinary and human antibiotics, 26 pathotypes were identified. The highest resistance was observed against Tetracyclines (92.3%) and Macrolides (80.8%), while almost all were susceptible to Penicillins, according to the limited use of beta-lactams on the farm. In total, 92.3% of pathotypes were multidrug resistant (MDRs). Two MDR pathotypes belonging to the t2802 spa type were isolated from both farmers and rabbits. Age categories harboured significantly different pathotypes (p = 0.019), while no association was found between pathotypes and lesions (p = 0.128) or sampling sites (p = 0.491). The antibiotic resistance was observed to increase with the time spent in the farm environment (age category). The selective pressure exerted by antibiotic use acted by giving advantage to more resistant strains rather than by lowering susceptibility to various drug categories within strains.
Variability inin vitrobiofilm production and antimicrobial sensitivity pattern amongPasteurella multocidastrains
BIOFOULING
Authors: Prajapati, Awadhesh; Chanda, Mohammed Mudassar; Dhayalan, Arul; Yogisharadhya, Revanaiah; Chaudhary, Jitendra Kumar; Mohanty, Nihar Nalini; Shivachandra, Sathish Bhadravati
Abstract
Biofilm production, hitherto an uncharacterized feature among circulatingPasteurella multocidastrains, was studied along with the antibiotic susceptibility pattern. On the basis of biofilm formation ability, all the strains were categorized into four groups under six different culture conditions: strong biofilm-forming (22%), moderate (19%), weak (51%), and non-adherent (7%). Strains from serogroups A and B formed significant biofilms in at least one culture condition whereas strains from serogroup D were unable to form biofilms. All strains were found to be susceptible to tetracycline. In addition, the correlation between diverse factors (host, capsule type, clinical condition and thetadDgene) as well as antimicrobial susceptibility in biofilm production were analyzed by Joint distribution models, and showed that enrofloxacin and azithromycin resistant strains were positively correlated with strong biofilm production.