Tetracycline ELISA Kit (Aquatic products)

Sample

Fresh eggs (chicken eggs, duck eggs, quail eggs), aquatic products (fish meat, shrimp meat)

Detection Method

cELISA

Intended Use

This product can be used for the qualitative and quantitative detection of tetracycline residues in fresh eggs (chicken eggs, duck eggs, quail eggs) and aquatic products (fish meat, shrimp meat).

Contents of Kit

1. 96-well microplate × 1 (pre-coated with conjugated antigen)
2. Standard solutions × 6 vials (1 mL/vial): 0 ppb, 0.2 ppb, 0.6 ppb, 1.8 ppb, 5.4 ppb, 16.2 ppb (1 ppb = 1 μg/L = 1 μg/kg)
3. High-concentration standard (1 mL/vial): 1 ppm
4. Enzyme-labeled secondary antibody concentrate (1 mL, red cap)
5. Antibody working solution (10 mL, green cap)
6. Substrate A (7 mL, white cap)
7. Substrate B (7 mL, red cap)
8. Stop solution (7 mL, yellow cap)
9. 20× Concentrated wash buffer (40 mL, transparent cap)
10. Reconstitution working solution (50 mL, blue cap)

Storage

Storage: 2–8°C
Shelf life: 12 months

Performance Characteristics

Accuracy: Aquatic products and fresh eggs: 100% ± 30%

Precision

Intra-assay and inter-assay coefficients of variation are both less than 10%.

Detection Limit

Detection Limit for aquatic products and fresh eggs:
1. Doxycycline: 5.0 ppb
2. Tetracycline: 2.0 ppb
3. Oxytetracycline: 2.0 ppb
4. Chlortetracycline: 4.0 ppb

Sensitivity

0.2 ppb

General Description

High-Performance Liquid Chromatography (HPLC) is the method most commonly used for detecting tetracycline residues; however, a drawback of this technique is that sample preparation is time-consuming and requires expensive laboratory equipment. This kit offers high accuracy and sensitivity, requires minimal technical expertise to operate, features a rapid detection time, and is capable of processing a large volume of samples. (Detection using this kit takes only 45 minutes.)

General Notes

High-Performance Liquid Chromatography (HPLC) is the method most commonly used for detecting tetracycline residues; however, a drawback of this technique is that sample preparation is time-consuming and requires expensive laboratory equipment. This kit offers high accuracy and sensitivity, requires minimal technical expertise to operate, features a rapid detection time, and is capable of processing a large volume of samples. (Detection using this kit takes only 45 minutes.)