Tetracyclines ELISA Kit (DEIA046)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Species Reactivity
N/A
Intended Use
This kit can be used in quantitative and qualitative analysis of tetracyclines residue in vaccine and cell culture.
Contents of Kit
1.Microtiter plate with 96 wells coated with antigen
2.Standard solutions(5×1ml/bottle)
0ng/ml, 0.2ng/ml, 0.6ng/ml, 1.8ng/ml, 5.4ng/ml
3.Spiking standard solution: (1ml/bottle) 1μg/ml
4.Concentrated enzyme conjugate 1ml ……….…red cap
5.Enzyme diluent 10ml ………………..……… green cap
6.Solution A 7ml…………...……………….……white cap
7.Solution B 7ml……………………………………red cap
8.Stop solution 7ml……………………….……yellow cap
9.20×concentrated wash solution 40ml…transparent cap
10.Sample diluent 50ml………………….……blue cap
Storage
Store the kit at 2-8 °C (36-46°F).
Storage period: 12 months.
Precision
CV of the ELISA kit is less than 10%.
Detection Range
0.2-5.4ng/ml
General Description
Tetracyclines residue in the production of biological samples may lead to severe allergic reactions in certain groups. Thus it is strictly controlled in many countries in the world.
This kit is a new product for drug residual detection based on ELISA technology, which is rapid, easy-to-use, and sensitive, and can considerably minimize operation errors and work intensity.
Standard Curve
1.To draw a standard curve: Take the absorbance value of standards as y-axis, semi logarithmic of the concentration of the tetracyclines standards solution (ng/ml) as x-axis.
2.The tetracyclines concentration of each sample (ng/ml), which can be read from the calibration curve, is multiplied by the corresponding dilution factor of each sample followed, and the actual concentration of sample is obtained.
Please notice: software has been developed for data reduction, which can be provided upon request.
Dilution factor of samples: according to your operation.

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References


Third-Generation Cephalosporin- and Tetracycline-ResistantEscherichia coliand Antimicrobial Resistance Genes from Metagenomes of Mink Feces and Feed

FOODBORNE PATHOGENS AND DISEASE

Authors: Agga, Getahun E.; Silva, Philip J.; Martin, Randal S.

American mink (Neovison vison) is a significant source of global fur production. Except for a few studies from Denmark and Canada reporting antimicrobial resistance in bacteria isolated from clinical cases, studies from the general mink population are scarce and absent in the United States. Mink feces (n = 42) and feed (n = 8) samples obtained from a mink farm were cultured for the enumeration and detection of tetracycline-resistant (TET (R))- and third-generation cephalosporin-resistant (TGC (R))-Escherichia coli. Isolates were characterized phenotypically for their resistance to other antibiotics and genotypically for resistance genes. TET (R) E. coliwere detected from 98% of feces samples (mean concentration = 6 log(10)) and from 100% of feed samples (mean concentration = 3.2 logs). Among TET (R) E. coliisolates, 44% (n = 41) of fecal- and 50% (n = 8) of feed isolates were multidrug resistant (MDR; resistance to >= 3 antimicrobial classes), and 96% (n = 49) of TET (R) isolates were positive fortet(A) and/ortet(B). TGC (R) E. coliwere detected from 95% of feces and 75% of feed samples with 78% (n = 40) of fecal isolates, and all six of the feed isolates were MDR. Nearly two-thirds (65%) of the TGC (R) E. coliisolates (n = 46) were positive forbla(CMY-2); the remaining 35% were positive forbla(CTX-M,)with thebla(CTX-M-14)being the predominant (75%,n = 16) variant detected. Metagenomic DNA was extracted directly from feces and feed samples, and it was tested for 84 antimicrobial resistance genes by using quantitative polymerase chain reaction (PCR) array; selected genes were also quantified by droplet digital PCR. The genes detected from the fecal samples belonged mainly to five antimicrobial classes: macrolide-lincosamide-streptogramin B (MLSB; 100% prevalence), TETs (88.1%), beta-lactams (71.4%), aminoglycosides (66.7%), and fluoroquinolones (47.6%). beta-Lactam, MLSB, and TET resistance genes were also detected from feed samples. Our study serves as a baseline for further studies and to streamline antimicrobial use in mink production in accordance with current regulations as in food animals.

Pharmacokinetics of doxycycline after oral administration of multiple doses in dogs

JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS

Authors: De Lucas, Jose Julio; Rodriguez, Casilda; San Andres, Maria Dolores; Sainz, Angel; Villaescusa, Alejandra; Garcia-Sancho, Mercedes; Rodriguez-Franco, Fernando; San Andres, Manuel I.

The aim of this study was to determine the pharmacokinetic parameters of doxycycline in dogs and assess the efficacy of an oral drug dosage regimen of 10 mg/kg daily for 28 days through Pharmacokinetic/Pharmacodynamic (PK/PD) target analysis based on Monte Carlo simulation, using previously published data for the zoonotic pathogen Staphylococcus pseudintermedius. After a multiple-dosage regimen, the accumulation index was 1.88 +/- 0.82. The Cmax(ss) and Cmin(ss) values were 5.18 +/- 1.81 mu g/ml and 1.91 +/- 1.35 mu g/ml, respectively. There were statistically significant differences for Cmax, Cmin at 24 hr, MRTt, AUCt and AUC infinity between days 1 and 28. The Cmin(ss) value was over the MIC of the principal pathogens, and Cmax(ss) was higher than the resistance values (>2 mu g/ml). For AUC/MIC indices of 12, 25 and 40, the cumulative fraction responses (CFR) were 94.01%, 69.55% and 60.86%, respectively; for an MIC value of 2 mu g/ml, the corresponding probability of target attainment (PTA) was 99.94%, 84.78% and 45.16%, respectively. Doxycycline was used against numerous localized infections in different organs and tissues. For the strains with MIC < 1 mu g/mL, PTA was close to 100%, even for the most demanding ones, specifically 94.98% for an index of 40% and 99.9% for an index of 25.

Yáñez-Sedeño, Paloma, et al. "Electrocatalytic (bio) platforms for the determination of tetracyclines." JOURNAL OF SOLID STATE ELECTROCHEMISTRY (2020).

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