The Streptomycin ELISA Kit is based on the competitive enzyme immunoassay for the detection of Streptomycin in vaccine and cell culture supernatant.
Contents of Kit
No.
Components
Size
Storage Conditions
1
Micro-well strips
12 × 8 wells
2-8°C
2
Standard solution
6 × 1 mL each
2-8°C
3
Enzyme conjugate
12 mL
2-8°C
4
Antibody working solution
7 mL
2-8°C
5
Substrate solution
6 mL × 2
2-8°C
6
Stop solution
7 mL
2-8°C
7
20× concentrated wash solution
50 mL
2-8°C
8
2× sample diluent
50 mL
2-8°C
Storage
Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Precision
Intra-assay: < 10% Inter-assay: < 10%
Detection Range
0-25.6ng/mL
General Description
Streptomycin is an antibiotic (antimycobacterial) drug, the first of a class of drugs called aminoglycosides to be discovered, and it was the first cure for tuberculosis. It is derived from the actinobacterium Streptomyces griseus. Streptomycin is a bactericidal antibiotic. Adverse effects of this medicine are ototoxicity, nephrotoxicity, fetal auditory toxicity, and neuromuscular paralysis. It is on the World Health Organization's List of Essential Medicines, a list of the most important medication needed in a basic health system.
Standard Curve
Citations
Publication ()
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Background
Streptomycin is an antibiotic that has played a significant role in the treatment of bacterial infections since its discovery in the mid-20th century. It was the first effective drug for the treatment of tuberculosis, a highly contagious and often deadly disease. Streptomycin is derived from the bacterium Streptomyces griseus and belongs to the class of antibiotics known as aminoglycosides. Streptomycin works by inhibiting bacterial protein synthesis, specifically targeting the 30S ribosomal subunit. It binds to the ribosome and disrupts the accurate reading of the genetic code, leading to the production of faulty proteins and ultimately inhibiting bacterial growth. This mechanism of action makes streptomycin effective against a broad spectrum of Gram-negative and some Gram-positive bacteria.
Figure 1. Mechanism of Action of streptomycin and mechanism of resistance associated with it. (Source: Sheikh, B. A. et al., 2021)
In addition to tuberculosis treatment, streptomycin has also been used in the management of other bacterial infections, such as certain strains of Mycobacterium, Yersinia pestis (the causative agent of bubonic plague), and various species of Enterobacteriaceae. While streptomycin has proven to be a valuable tool in combating bacterial infections, it is not without potential side effects and toxicities. These can include ototoxicity (hearing loss), nephrotoxicity (kidney damage), and neurotoxicity (nerve damage). Therefore, careful monitoring and consideration of the risks and benefits are necessary when using streptomycin. Moreover, the overuse and misuse of streptomycin have contributed to the emergence of antibiotic resistance, emphasizing the need for accurate and reliable methods to detect and quantify streptomycin residues in different samples. Compared with traditional methods, the Streptomycin ELISA Kit is simple, fast, and sensitive, and is suitable for the detection of large quantities of samples. This kit (DEIA020) is a competitive ELISA assay for the quantitative measurement of streptomycin in vaccine and cell culture supernatant. The density of color is proportional to the amount of streptomycin captured from the samples.
Alternative Names
Streptomycin ELISA Streptomycin (SM) ELISA Streptomycin ELISA Kit SM ELISA Kit ELISA Kit for SM (Streptomycin)
References
1. Sheikh B A, et al. Development of new therapeutics to meet the current challenge of drug resistant tuberculosis. Current Pharmaceutical Biotechnology. 2021, 22(4): 480-500.
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References
A structural basis for streptomycin-induced misreading of the genetic code
During protein synthesis, the ribosome selects aminoacyl-transfer RNAs with anticodons matching the messenger RNA codon present in the A site of the small ribosomal subunit. The aminoglycoside antibiotic streptomycin disrupts decoding by binding close to the site of codon recognition. Here we use X-ray crystallography to define the impact of streptomycin on the decoding site of the Thermus thermophilus 30S ribosomal subunit in complexes with cognate or near-cognate anticodon stem–loop analogues and messenger RNA. Our crystal structures display a significant local distortion of 16S ribosomal RNA induced by streptomycin, including the crucial bases A1492 and A1493 that participate directly in codon recognition. Consistent with kinetic data, we observe that streptomycin stabilizes the near-cognate anticodon stem–loop analogue complex, while destabilizing the cognate anticodon stem–loop analogue complex. These data reveal how streptomycin disrupts the recognition of cognate anticodon stem–loop analogues and yet improves recognition of a near-cognate anticodon stem–loop analogue.
Determination of streptomycin residues in animal-derived foods by a reliable and accurate enzyme-linked immunosorbent assay
A highly reliable and accurate indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed for the detection of streptomycin (STR) in animal-derived foods. The half-maximum inhibition concentration (IC50) of the icELISA for STR was 2.00 ng mL-1 and the limit of detection (LOD, calculated as the IC15 value) was 0.24 ng mL-1. The cross-reactivities with other aminoglycoside antibiotics were very low except for dihydrostreptomycin (95.50%). The recoveries for all samples at five levels (15, 30, 120, 300, 600 ng g-1) ranged from 71.32% to 106.94%. The coefficients of variation of intra- and inter-assays were below 14%. There was a good correlation (R2 = 0.9854) between the data determined by this ELISA and the commercial kit. The analysis of variance of the recoveries showed the sufficiently high stability of the assay to STR in eight animal-derived foods (chicken, pork, chicken liver, pig liver, pig kidney, fish, shrimp, and cow milk). The ELISA allows for a rapid, low-cost, sensitive and accurate determination of STR residues in animal-derived foods.
COA
Publication 
Figure 1. Mechanism of Action of streptomycin and mechanism of resistance associated with it.
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