Intended Use
This kit can be used in quantitative and qualitative analysis of streptomycin residue in vaccine etc.
Contents of Kit
1.Microtiter plate with 96 wells coated with antibody
2.Standard solutions (6 bottles×1ml/bottle)
0, 0.05, 0.15, 0.45, 1.35, 4.05ng/ml
3.Spiking standard solution: (1ml/bottle) 1μg/ml
4.Concentrated Enzyme conjugate 1ml……….red cap
5.Enzyme conjugate diluent 10ml…………. green cap
6.Substrate solution A 7ml………….………white cap
7.Substrate solution B 7ml……………………red cap
8.Stop solution 7ml………………....………yellow cap
9.20×Concentrated wash solution 40ml………transparent cap
10.2×Sample diluent 50ml……blue cap
Storage
Storage condition: 2-8°C .
Storage period: 12months.
Precision
CV of the ELISA kit all less than 10%.
Accuracy
85±10%
Detection Range
0.05-4.05ng/ml
General Description
Streptomycin is an aminoglycoside antibiotic, which is broadly applied in animal disease treatment. For it has neurotoxicity and kidney toxicity, its residue in animal-derived food is harmful to human; it is strictly controlled in use in EU, US and China. At present, ELISA is the common approach in supervision and control of streptomycin drug.
This kit is a new product for drug residual detection based on ELISA technology, which only costs 45 min in each operation and can considerably minimize operation errors and work intensity.
Standard Curve
To draw a standard curve: Take the absorbance value of standards as y-axis, semi logarithmic of the concentration of the streptomycin standards solution (ppb) as x-axis.
The streptomycin concentration of each sample (ppb), which can be read from the calibration curve, is multiplied by the corresponding dilution factor of each sample followed, and the actual concentration of sample is obtained.
Please notice:
Special software has been developed for all data analysis, which can be provided on request.
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