Streptomycin Rapid Test (Milk)

The aim of the study was to evaluate the cytotoxicity of tulathromycin (macrolide antibiotic) on BHK-21 An30 cells and to determine the effect on FMD (Serotypes; A/TUR/11, O/TUR/07, Asia-1/TUR/15) virus propagation in vitro. In the result of MTT tests and cell culture studies, the non-toxic upper concentration of the tulathromycin limit for BHK-21 An30 cell culture was determined as 30 mu g/ml in terms of cell morphologies and numbers. The mean FMD 146S virus particle values produced in BHK-21 An30 cell cultures containing 30 fig/nil of tulathromycin were found to be 0.47, 0.57 and 0.25 mu g/ml; the mean infective titer determined as 10(7.03), 10(6.23) and 10(7.40) pfu/ml for A/TUR/11, O/TUR/07 and Asia-1/TUR/15, respectively. In the FMD virus cultures produced with medium containing penicillin-streptomycin, the mean values of 146S virus particles were determined to be A/TUR/11, O/TUR/07 and the Asia-1/TUR/15 serotypes were 0.50, 0.55, and 0.32 mu g/ml, respectively, while the mean infective titers were 10(7.46), 10(6.62) and 10(7)(.7)(3) pfu/ml, respectively. As a result, it was concluded that tulathromycin can be used as an antibiotic up to 30 mu g/ml in a BHK-21 An30 cell culture and in the production media of the FMD virus A, O, and Asia-1 serotypes.

Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs-CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV-visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000-1,1000 and 120-480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.