Detection Method
Competitive ELISA
Intended Use
The kit is intended for the quantitative determination of S100B (S100A1B + S100BB) in serum.
Contents of Kit
1. Streptavidin Microtiter Plate - 1 Plate
2. S100 Calibrators 6 vials, lyophilized
3. Biotin Anti-S100 - 1 x 15 mL
4. Tracer, HRP Anti-S100 - 1 x 0. 75 mL
5. Tracer Diluent - 1 x 15 mL
6. TMB HRP-Substrate - 1 x 12 mL
7. STOP Solution - 1 x 15 mL
8. Wash Concentrate - 1 x 50 mL
Storage
Store the kit at 2-8°C. Do not freeze. For more detailed information, please download the following document on our website.
Detection Range
The kit measures concentrations between 10 and 3500 ng/L. If S100B concentrations above the measuring range are to be expected, it is recommended to dilute samples with normal human serum prior to analysis. NOTE: The serum used for dilution should also be measured in order to determine the endogenous S100B concentration.
Citations
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Sviatoha, V; Tani, E; et al. Immunohistochemical analysis of the S100A1, S100B, CD44 and Bcl-2 antigens and the rate of cell proliferation assessed by Ki-67 antibody in benign and malignant melanocytic tumours. MELANOMA RESEARCH 20:118-125(2010).
Boni, R; Burg, G; et al. Immunohistochemical localization of the Ca2+ binding S100 proteins in normal human skin and melanocytic lesions. BRITISH JOURNAL OF DERMATOLOGY 137:39-43(1997).
COA
Certificate of Analysis
