Human KITLG peptide

Name: Human KITLG peptide
SKU: DAG329
Rating: 5 (1 reviews)

Recombinant Human KITLG peptide

COA

SDS

Datasheet

Summary

Product Overview

RecombinantHuman Stem Cell Factor is a single, non-glycosylated, polypeptide chain containing165 amino acids and having a molecular weight of 18,409 Da. The sequence ofthe first five N-terminal amino acids was determined to beMet-Glu-Gly-Ile-Cys. Contains

Nature

Recombinant

Tag/Conjugate

Unconjugated

Alternative Names

SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7

Format

Purified, Lyophilized; Reconstitute using sterile deionized water to a concentration 100μg/ml. Further dilutions can bemade in other aqueou buffers.

Concentration

1 mg/ml (OD280nm, E0.1% = 0.52) (prior tolyophilization)

Buffer

Lyophilized from 10mM Acetic acid

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

4254

Antigen Description

StemCell Factor(also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that bindsto the c-Kit receptor (CD117). SCF can exist both as a transmembrane proteinand a soluble protein. This cytokine plays an important role in hematopoiesis(formation of blood cells), spermatogenesis, and melanogenesis.

Keywords

KITLG; KIT ligand; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; kit ligand; c-Kit ligand; steel factor; stem cell factor; mast cell growth factor; familial progressive hyperpigmentation 2;

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Documents

pdf COA

pdf SDS

pdf Datasheet

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References

Predicting Gonadal Germ Cell Cancer in People with Disorders of Sex Development; Insights from Developmental Biology

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Authors: Looijenga, Leendert H. J.; Kao, Chia-Sui; Idrees, Muhammad T.

Abstract

The risk of gonadal germ cell cancer (GGCC) is increased in selective subgroups, amongst others, defined patients with disorders of sex development (DSD). The increased risk is due to the presence of part of the Y chromosome, i.e., GonadoBlastoma on Y chromosome GBY region, as well as anatomical localization and degree of testicularization and maturation of the gonad. The latter specifically relates to the germ cells present being at risk when blocked in an embryonic stage of development. GGCC originates from either germ cell neoplasia in situ (testicular environment) or gonadoblastoma (ovarian-like environment). These precursors are characterized by presence of the markers OCT3/4 (POU5F1), SOX17, NANOG, as well as TSPY, and cKIT and its ligand KITLG. One of the aims is to stratify individuals with an increased risk based on other parameters than histological investigation of a gonadal biopsy. These might include evaluation of defined susceptibility alleles, as identified by Genome Wide Association Studies, and detailed evaluation of the molecular mechanism underlying the DSD in the individual patient, combined with DNA, mRNA, and microRNA profiling of liquid biopsies. This review will discuss the current opportunities as well as limitations of available knowledge in the context of predicting the risk of GGCC in individual patients.

Fibroblast Growth Factor 7 Stimulates In Vitro Growth of Oocytes Originating From Bovine Early Antral Follicles

MOLECULAR REPRODUCTION AND DEVELOPMENT

Authors: Cho, Joon-Ho; Itoh, Takehiro; Sendai, Yutaka; Hoshi, Hiroyoshi

Abstract

Essential factors required for growing oocytes derived from bovine early antral follicles and their mechanisms of action are poorly understood. Fibroblast growth factor 7 (FGF7) is a member of the heparin-binding FGF family with a distinctive pattern of target-cell specificity. The effect of FGF7 on the stimulation of oocyte growth in a culture of cumulus-oocyte complexes with granulosa cells (COCGs, oocyte diameter; 90-100 mu m) was investigated. The oocyte diameter of COCGs was increased significantly in the FGF7-containing medium (10 ng/ ml; 117.2 +/- 3.2 mu m, 50 ng/ml; 116.5 +/- 3.5 mu m) compared to the control (0 ng/ml; 110.5 +/- 2.8 mu m) after 16 days. However, there was no stimulatory effect of FGF7 on the proliferation of cumulus-granulosa cells. The FGF7 receptor, fibroblast growth factor receptor 2IIIb (FGFR2IIIb), was detected in cumulus-granulosa cells from COCGs. Messenger RNA expression of FGFR2IIIb was induced to cumulus-granulosa cells by FGF7. The mRNA expression levels of KIT ligand (KITLG), KIT (KIT), growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) in the cultured COCGs were determined in FGF7-treated (10 ng/ml) cultures using real time RTPCR analysis. The levels of KITLG and KIT, but not GDF9 and BMP15 mRNA expression were stimulated by FGF7. Furthermore, neutralizing antibody for KIT attenuated the stimulatory action of FGF7 on the oocyte growth. These results strongly suggest that FGF7 may be an important regulator for oocyte growth and its action is mediated via the KIT/KITLG signaling pathway. Mol. Reprod. Dev. 75: 1736-1743, 2008. (c) 2008 Wiley-Liss, Inc.