Human KIT Ligand

Name: Human KIT Ligand
SKU: DAG357
Rating: 5 (1 reviews)

Human KIT Ligand, recombinant protein from E. coli

COA

SDS

Datasheet

Summary

Product Overview

Ahematopoietic growth factor that exerts its activity at the early stages ofhematopoiesis. KITLG stimulates the proliferation of myeloid, erythroid, andlymphoid progenitors in bone marrow cultures and has been shown to actsynergistically with colony stimu

Nature

Recombinant

Tag/Conjugate

Unconjugated

Format

Purified, Lyophilized. Reconstitute in 10mM Acetic Acid to a concentration of0.1-1.0 mg/ml. This solution can be diluted into water or othe bufferedsolutions or stored at –20° C for future use.

Concentration

Not applicable.

Buffer

Not applicable.

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Antigen Description

StemCell Factor (also known as SCF, kit-ligand, KL, or steel factor) is acytokine that binds to the c-Kit receptor (CD117). SCF can exist both as atransmembrane protein and a soluble protein. This cytokine plays an importantrole in hematopoiesis (formation of blood cells), spermatogenesis, andmelanogenesis.

Keywords

KITLG; KIT ligand; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; kit ligand; c-Kit ligand; steel factor; stem cell factor; mast cell growth factor; familial progressive hyperpigmentation 2;

Citations

Have you cited DAG357 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Fibroblast Growth Factor 7 Stimulates In Vitro Growth of Oocytes Originating From Bovine Early Antral Follicles

MOLECULAR REPRODUCTION AND DEVELOPMENT

Authors: Cho, Joon-Ho; Itoh, Takehiro; Sendai, Yutaka; Hoshi, Hiroyoshi

Abstract

Essential factors required for growing oocytes derived from bovine early antral follicles and their mechanisms of action are poorly understood. Fibroblast growth factor 7 (FGF7) is a member of the heparin-binding FGF family with a distinctive pattern of target-cell specificity. The effect of FGF7 on the stimulation of oocyte growth in a culture of cumulus-oocyte complexes with granulosa cells (COCGs, oocyte diameter; 90-100 mu m) was investigated. The oocyte diameter of COCGs was increased significantly in the FGF7-containing medium (10 ng/ ml; 117.2 +/- 3.2 mu m, 50 ng/ml; 116.5 +/- 3.5 mu m) compared to the control (0 ng/ml; 110.5 +/- 2.8 mu m) after 16 days. However, there was no stimulatory effect of FGF7 on the proliferation of cumulus-granulosa cells. The FGF7 receptor, fibroblast growth factor receptor 2IIIb (FGFR2IIIb), was detected in cumulus-granulosa cells from COCGs. Messenger RNA expression of FGFR2IIIb was induced to cumulus-granulosa cells by FGF7. The mRNA expression levels of KIT ligand (KITLG), KIT (KIT), growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) in the cultured COCGs were determined in FGF7-treated (10 ng/ml) cultures using real time RTPCR analysis. The levels of KITLG and KIT, but not GDF9 and BMP15 mRNA expression were stimulated by FGF7. Furthermore, neutralizing antibody for KIT attenuated the stimulatory action of FGF7 on the oocyte growth. These results strongly suggest that FGF7 may be an important regulator for oocyte growth and its action is mediated via the KIT/KITLG signaling pathway. Mol. Reprod. Dev. 75: 1736-1743, 2008. (c) 2008 Wiley-Liss, Inc.

Isolation and molecular characterization of spermatogonia from male Sprague-Dawley rats exposed in utero and postnatally to dibutyl phthalate or acrylamide

TOXICOLOGY MECHANISMS AND METHODS

Authors: Souza, Nathalia P.; Arnold, Lora L.; Pennington, Karen L.; Nascimento e Pontes, Merielen G.; Miot, Helio A.; de Camargo, Joao Lauro, V; Cohen, Samuel M.

Abstract

The increased incidence of testicular disorders in young men and the possible influence of environmental chemicals, such as dibutyl phthalate (DBP) and acrylamide (AA), requires experimental models for identifying modes of action. Most published reproductive toxicologic studies use RNA samples from the total testis to evaluate testicular gene expression; however, analyses of isolated cell types could provide a more specific tool. Among testicular germ cells, spermatogonia are critical since they represent the onset of spermatogenesis. This study aimed, (1) to establish a technique for spermatogonia isolation; (2) to apply this isolation technique to verify possible gene expression alterations (Pou5f1, Kitlg, Mki-67, Bak1 and Spry4) in prepubertal post-natal day, (PND24) and pubertal (PND45) testes after in utero and postnatal exposure to DBP or AA. The technique was efficient for isolation of a majority of spermatogonia. In utero DBP exposure led to reduced litter body weight at birth, reduced anogenital distance of male pups on PND4, and increased frequency of male nipple retention on PND14 compared to controls. DBP-exposed relative testes weights were reduced only at PND24 compared to control but they did not differ at PND45. DBP-exposed animals showed reduced expression levels of Pou5f1 and Mki67 on PND24, and reduced expression of Pou5f1 and Spry4 on PND45. AA exposure reduced expression of Pou5f1, Mki67, and Spry4 at PND45 although not significantly. Our results suggest that DBP acts by reducing cell proliferation and impairing differentiation in prepubertal and pubertal testes.