HCV type a+b Nonstructural Protein 4 [Rhodamine] (DAG1423)

HCV type a+b Nonstructural Protein 4 [Rhodamine], recombinant protein from E. coli Datasheet

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Product Overview
Recombinant HCV NS4 rhodamine labeled protein containing amino acids 1658-1863 was expressed in E. coli and purified by proprietary chromatographic technique.
Nature
Recombinant
Tag/Conjugate
Rhodamine
Purity
> 95% pure as determined by 10% PAGE (coomassie staining).
Buffer
20mM Tris-Hcl pH 8, 8M urea and 10mM B-ME.
Storage
2-8°C short term, -20°C long term
Introduction
HCV is a positive, single-stranded RNAvirus in the Flaviviridae family. The genome is approximately 10, 000nucleotides and encodes a single polyprotein of about 3, 000 amino acids.HCVis responsible for a large proportion of worldwide chronic viral hepatitides.Most of these infections develop into chronic hepatitis, which oftenprogresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis Cinfection.The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is arelatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
Antigen Description
NS4B is a small (27 kDa) hydrophobic integral membrane protein with 4 transmembrane domains. It is located within the endoplasmic reticulum and play an important role for recruitment of other viral proteins. It induces morphological changes to the endoplasmic reticulum forming a structure termed the membranous web.
Keywords
HCV; HCV NS4; Hepatitis C Virus nonstructural antigen 4; Non structural protein 4A; Non structural protein 4B; NS4A; NS4B; Flaviviridae; Hepacivirus; Hepatitis C virus; p27; p8; Hepatitis C Virus NS4; HCV NS4 Genotype 5; Hepatitis C Virus NS4 Genotype 5

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