Recombinant HCV type 1a core protein [His]

Nature

Recombinant

Tag/Conjugate

His

Molecular Weight

24 kDa

Purity

≥95% as determined by SDS-PAGE

Format

Liquid or Lyophilized powder

Buffer

Lyophilized from PBS with 8M Urea

Preservative

None

Storage

Store at -20°C to -80°C

Reconstitution

Reconstitute the protein with 100 μL of deionized water

Introduction

HCV core protein, derived from N-terminal 191 amino acids of the polyprotein, is the only HCV protein to directly interact with and encapsulate HCV genomic RNA and form nucleocapsids. The HCV core protein consists of three domains: the highly cationic domain 1, with approximately 117 amino acids, is the major domain to associate with HCV genomic RNA and to form nucleocapsids; the hydrophobic domain 2 is responsible for association with lipid droplets crucial for infectious virus particle productions; the domain 3, also hydrophobic, is the endoplasmic reticulum (ER) anchoring domain. The majority of HCV core protein is found to locate at the ER, mitochondria, and lipid droplets in cytoplasm.

Keywords

Hepatitis C virus; HCV; Core protein