HCV Core Antigen ELISA Kit

Sample

culture medium

Detection Method

sELISA

Intended Use

CD HCV Core Antigen ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the HCV core protein. The kit has detection sensitivity limit of 1 ng /mL HCVcAg. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and HCV samples.

Contents of Kit

Box 1 (shipped at room temperature)
1. Anti-HCVcAg Antibody Coated Plate: One strip well 96-well plate.
2. FITC-Conjugated Anti-HCVcAg Monoclonal Antibody: One 20 μL vial.
3. HRP-Conjugated Anti-FITC Monoclonal Antibody: One 20 μL vial.
4. Assay Diluent: One 50 mL bottle.
5. Triton X-100 Solution: One 15 mL bottle containing 5% Triton X-100 in TBS.
6. 10× Wash Buffer: One 100 mL bottle.
7. Substrate Solution: One 12 mL amber bottle.
8. Stop Solution: One 12 mL bottle.
Box 2 (shipped on blue ice packs)
1. Recombinant HCVcAg Standard: One 100 μL vial of 10 μg/mL recombinant HCV Core Antigen in 8 M Urea containing BSA.

Storage

Upon receiving, aliquot and store recombinant HCVcAg Standard at -20°C and avoid freeze/thaw. Store all other components at 4°C.

General Description

Hepatitis C is an infection of the liver caused by the hepatitis C virus. HCV is transmitted by exposure to infectious blood; forms of transmission include unprotected sexual activity, blood transfusion, mother-to-infant transmission, or occupational exposure to blood. The acute illness causes liver inflammation, liver fibrosis, vomiting and jaundice, while chronic HCV infection often leads to liver cirrhosis and failure.
An estimated 270-300 million people worldwide have been infected with hepatitis C. At least 75% of people infected will develop chronic hepatitis C and have it the rest of their lives.
Diagnosis of chronic hepatitis C virus (HCV) infection has long been based on HCV serology and detection of HCV antibodies. With the development of therapies for chronic HCV infection, including interferon and ribavirin, quantitative detection of HCV has been used increasingly as the most important marker for monitoring HCV titer, disease progression, and assessing antiviral treatment.
Several assays for the quantitative measurement of HCV DNA have been developed, such as PCRbased nucleic acid amplification assays. However, these methods tend to be cumbersome and expensive.

Standard Curve