Detection Method
Sandwich ELISA
Intended Use
The Rat Active PAI1 ELISA Kit is intended for the quantitative determination of active plasminogen activator inhibitor type 1 in rat plasma.
Contents of Kit
1. uPA coated plate
2. 10X Wash Buffer
3. Rat PAI-1 activity Standard
4. Anti-rat PAI-1 primary antibody
5. Anti-rabbit horseradish peroxidase secondary antibody
6. TMB substrate solution
Storage
All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -70°C for later use. DO NOT
freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.
Precision
Intra Assay Precision: High 3.5%, Medium 4.9%, Low 2.8% (calculated by running 24 reps of each concentration in an assay).
Citations
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Sigdel, TK; Kaushal, A; et al. Shotgun proteomics identifies proteins specific for acute renal transplant rejection. PROTEOMICS CLINICAL APPLICATIONS 4:32-47(2010).
Semler, O; Netzer, C; et al. First use of the RANKL antibody denosumab in Osteogenesis Imperfecta Type VI. JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS 12:183-188(2012).