RNP/Sm Antibody IgG ELISA Kit

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Anti-RNP/Sm ELISA is a test system for the quantitative measurement of IgG class autoantibodies against RNP/Sm in human serum or plasma.

Contents of Kit

1.MICROPLATE, 1, One divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
2.Calibrator A 0 U/mL, 1x1.5 mL, containing serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%). yellow. Ready to use.
3.Calibrator B 12.5 U/mL, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
4.Calibrator C 25 U/mL, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
5.Calibrator D 50 U/mL, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
6.Calibrator E 100 U/mL, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
7.Calibrator F 200 U/mL, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
8.Control positive, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
9.Control negative, 1x1.5 mL, containing RNP/Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
10.DILUENT, Sample Buffer P, 20 mL, containing PBS, BSA, detergent, preservative NaN₃ 0.09%, yellow, concentrate (5x).
11.Enzyme Conjugate, 15 mL, containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative Proclin 0.05 %, light red. Ready to use.
12.TMB Substrate, 15 mL, containing 3,3', 5,5'-Tetramethylbenzidin, colorless. Ready to use.
13.Stop Solution, 15 mL, contains acid. Ready to use.
14.Wash Buffer, 20 mL, containing Tris, detergent, preservative NaN₃ 0.09%; 50X conc.
15.Instruction for Use, 1
16.Certificate of Analysis, 1

Storage

1.Store lest kit at 2°C - 8°C in the dark.
2.Do not expose reagents to heat, sun, or strong light during storage and usage.
3.Store microplate sealed and desiccated in the clip bag provided.
4.Shelf life of the unopened test kit is 18 months from day of production. Unopened reagents are stable of the kit. See labels for Individual batch.
5.Diluted Wash Buffer and Sample Buffer are stable for at least 30 days when stored at 2°C - 8°C.
6.We recommend consumption on the same day.

Detection Range

0-200 U/mL

Detection Limit

1U/mL