Anti-Sm/RNP ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For the detection and semi-quantitation of antibodies against the Sm/RNP antigen in serum as an aid in the diagnosis of autoimmune disease.

Contents of Kit

1. Microwells Coated with RNP/Sm Antigen 12×8×1
2. Sample Diluent: 1 bottle (ready to use) 22 ml
3. Calibrator: 1 Vial (ready to use) 1 ml
4. Positive Control: 1 vial (ready to use) 1 ml
5. Negative Control: 1 vial (ready to use) 1 ml
6. Enzyme conjugate: 1 bottle (ready to use) 12 ml
7. TMB Substrate: 1 bottle (ready to use) 12 ml
8. Stop Solution: 1 bottle (ready to use) 12 ml
9. Wash Concentrate 20×: 1 bottle 25 ml

Storage

1. Store the kit at 2-8°C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun or strong light.

General Description

Systemic rheumatic disease is characterized by the presence of circulating autoantibodies that are widely reactive with both nuclear and cytoplasmic antigens. The RNP and Sm antigens consist of portions of the U1 RNA and nine associated polypeptides. Antibodies to Sm/RNP are detected in up to 40% of patients with systemic lupus erythematosus (SLE) either alone or in conjunction with Sm antibodies. The RNP antigen is very closely associated with the Sm antigen and is designated the Sm/RNP complex. In contrast to anti-Sm, anti-RNP is found in patients with a variety of rheumatic diseases including scleroderma, rheumatoid arthritis, discoid lupus, polymyositis and Sjogren's Syndrome. High titers of anti-RNP, in the absence of anti-Sm, are correlated with mixed connective tissue disease (MCTD). Until recently, many laboratories used Immunodiffusion (ID), Counterimmunoelectro-phoresis, and hemagglutination to detect RNP antibodies. However, these methods are timeconsuming and cumbersome to perform and are insensitive relative to newer methods. Enzyme immunoassay (EIA) has advantages over the ID method in sensitivity, specificity, ease of automation, and testing turnaround time.

General Notes

For the detection and semi-quantitation of antibodies against the Sm/RNP antigen in serum as an aid in the diagnosis of autoimmune disease.