Penicillin G [KLH]

Product Overview

Penicillin G, KLH-conjugate

Target

PCN

Nature

Synthetic

Tag/Conjugate

KLH

Format

Liquid

Concentration

Approximately 2.0 mg/mL KLH

Buffer

Supplied in 20 mM PBS, pH 7.4

Preservative

None

Storage

Keep below -20°C for up to 1 year. Avoid repeated freeze-and-thaw. For short term storage (< 3 weeks) keep at 4°C.

Introduction

Penicillin is a group of Beta-lactam antibiotics used in the treatment of bacterial infections caused by susceptible, usually Gram-positive, organisms. beta-lactam antibiotics work by inhibiting the formation of peptidoglycan cross-links in the bacterial cell wall, which results in cytolysis.

Antigen Description

The penicillin G and KLH (keyhole limpet hemocyanin) (10 mg each) are conjugated by EDC method in 0.1 M MES pH 5.0. The carboxyl group in the penicillin G is directly linked to an amine group in the KLH without any linker by EDC conjugation method. Given the molecular weights of penicillin G and KLH are 334. 4Da and 8,000 – 9,000 kDa, respectively, the molar ratio of penicillin G:KLH in the conjugation solution 23923-26913:1. The resultant conjugation solution is then buffer-exchanged with 20 mM PBS, pH 7.4. The number of penicillin G that is actually conjugated to each KLH molecule is not determined. Note: Due to its high molecular weight and its tendency to form aggregates, the conjugate is not completely soluble in the buffer that it is in. Therefore, it is strongly recommended to vigorously vortex immediately prior to aliquot or use.

Keywords

Ampicillin; Benzylpenicillin; PCN; Penicillin G; Phenoxymethylpenicillin; Penicillin