Sample
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Detection Method
Competitive ELISA
Intended Use
Rat Orexins ELISA Kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the Orexins. This ELISA kit is for research use only, not for therapeutic or diagnostic applications.
Contents of Kit
1. MICROTITER PLATE: 96 wells
2. ENZYME CONJUGATE: 6.0 mL or 10 ml
3. STANDARD A-F: 1 vial each
4. SUBSTRATE A: 6 mL
5. SUBSTRATE B: 6 mL
6. STOP SOLUTION: 6 mL
7. WASH SOLUTION (100 x): 10 mL
8. BALANCE SOLUTION: 3 mL
Storage
All components of this kit are stable at 2-8°C until the kit's expiration date.
Detection Range
50 -1000 pg/mL
Citations
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Blouin, AM; Thannickal, TC; et al. Narp immunostaining of human hypocretin (orexin) neurons - Loss in narcolepsy. NEUROLOGY 65:1189-1192(2005).
Tanaka, S; Honda, Y; et al. Detection of autoantibodies against hypocretin, hcrtr1, and hcrtr2 in narcolepsy: Anti-hcrt system antibody in narcolepsy. SLEEP 29:633-638(2006).
COA
Certificate of Analysis
