Species Reactivity
human, bovine, canine, mouse, ovine, porcine, rat
Intended Use
The Orexin A EIA Kit is a competitive immunoassay for in vitro quantitative measurement of Orexin A in human, bovine, canine, mouse, ovine, porcine, rat serum or plasma.
Contents of Kit
1. EIA buffer concentrate
2. 96-well immunoplate with acetate plate sealer
3. Anti serum (lyophilized powder)
4. Standard (1 ug lyophilized powder)
5. Biotinylated tracer (lyophilized powder)
6. Sreptavidin-HRP
7. TMB substrate solution
8. Stop solution
9. Standard diluent 8ml
10. Datasheet
11. Protocol
Storage
Lyophilized components and standard diluent at a constant -20°C, The remaining components should be stored in the refrigerator (2-4°C)
Performance Characteristics
AVERAGE IC50: 0.33 ng/ml
Detection Range
0-25 ng/ml
Citations
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| Product Name |
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Blouin, AM; Thannickal, TC; et al. Narp immunostaining of human hypocretin (orexin) neurons - Loss in narcolepsy. NEUROLOGY 65:1189-1192(2005).
Bourgin, P; Huitron-Resendiz, S; et al. Hypocretin-1 modulates rapid eye movement sleep through activation of locus coeruleus neurons. JOURNAL OF NEUROSCIENCE 20:7760-7765(2000).
COA
Certificate of Analysis
