Sample
Cell culture supernate, serum, and plasma samples
Intended Use
For the development of sandwich ELISAs to measure natural and recombinant mouse S100 Calcium Binding Protein A8 and S100 Calcium Binding Protein A9 Heterodimer (S100A8/S100A9 Heterodimer). This package insert must be read in its entirety before using this product. Refer to the Certificate of Analysis for component concentrations as they may vary. For research use only. Not for use in diagnostic procedures.
This kit contains sufficient materials to run ELISAs on at least five 96 well plates, provided the following conditions are met:
The reagents are prepared as described in this package insert.
The assay is run as described in the General ELISA Protocol.
The recommended microplates, buffers, diluents, substrates, and solutions are used.
Contents of Kit
1. Mouse S100A8/S100A9 Heterodimer Capture Antibody: 1 vial
2. Mouse S100A8/S100A9 Heterodimer Detection Antibody: 1 vial
3. Mouse S100A8/S100A9 Heterodimer Recombinant Standard: 2 vial
4. Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP): 1 vial
Storage
Store the unopened kit at 2-8 °C. Do not use past kit expiration date.
Detection Range
125.0 - 8,000 pg/mL
General Description
S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca2+-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca2+-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.
Standard Curve
This standard curve is only for demonstration purposes. A standard curve should be generated for each set of samples assayed.
Citations
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COA
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