Melatonin direkt Saliva (Non-Extraction) ELISA Kit (DEIABL355)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
saliva
Species Reactivity
Human
Intended Use
Enzyme immunoassay for the direct, quantitative determination of melatonin in human saliva.
Contents of Kit
Microtiter Plate: 1 x 12x8
Standard A-F: 1 x 10 mL ,1 x 5 x 1 mL
Control 1+2: 1 x 2 x 1 mL
Melatonin Antiserum: 1 x 7 mL
Melatonin Biotin: 1 x 12 mL
Enzyme Conjugate: 1 x 12 mL
Wash Buffer Concentrate (20x): 1 x 50 mL
TMB Substrate Solution: 1 x 15 mL
TMB Stop Solution: 1 x 15 mL
Adhesive Foil: 3 x
Storage
2-8°C
Precision
Detection Range
0.5 - 50 pg/ml
Sensitivity
0.3 pg/mL
General Description
Enzyme immunoassay for the direct, quantitative determination of melatonin in human saliva.The pineal gland ("corpus pineale") has been called a neuroendocrine transducer because of its important role in photoperiodism. The major hormone of the pineal gland is N-acetyl-5-methoxy-tryptamine or melatonin which is synthezised from the amino acid tryptophane. Melatonin has the highest levels in plasma during nighttime. Its characteristic nocturnal surge appears to encode temporal information such as length of night. Regulation of the melatonin secretion is under neural control. Sympathetic innervation seems to play a major role via its release of noradrenaline. Altered patterns and/or levels of melatonin secretion have been reported to coincide with sleep disorders, "jet lag", depression, stress, schizophrenia, hypothalamic amenorrhea, pregnancy, anorexia nervosa, some forms of cancer, immunological disorders as well as control of sexual maturation during puberty. Most of the circulating melatonin is metabolized in the liver to 6-hydroxymelatonin and subsequently to 6- sulfatoxymelatonin which is excreted into the urine.
Standard Curve

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