Salivery Melatonin ELISA Kit

Name: Salivery Melatonin ELISA Kit
SKU: DEIA-XY58
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum or plasma

Species Reactivity

Human

Detection Method

ELISA

Intended Use

The Melatonin Enzyme Immunoassay Kit is a competitive immunoassay specifically designed and validated for the quantitative measurement of salivary Melatonin. It is not intended for diagnostic use. It is intended only for research use in humans and some animals. CD has not validated this kit for serum or plasma samples.

Please read the complete kit insert before performing this assay. Failure to follow kit procedure and recommendations for saliva collection and sample handling may result in unreliable values.

Contents of Kit

1. Microtitre Plate Coated with rabbit anti-Melatonin monoclonal antibodies. 1/96 well
2. Melatonin Standard 50 pg/mL, in a saliva-like matrix. Serially dilute before use according to Reagent Preparation.
Contains: Melatonin, buffer, preservative. 1 vial / 1.5 mL.
3. Melatonin Controls High and Low, in a saliva-like matrix. High control is ready to use. Low control formulated for stability when stored at 4°C. Prepare before use according to Reagent Preparation.
Contains: Melatonin, buffer, preservative. 2 vials / 1 mL High, 200 μL Low.

4. Melatonin Enzyme Conjugate Concentrate. Dilute before use with Melatonin Assay Diluent. (See step 5 of Procedure).
Contains: Melatonin conjugated to HRP, buffer, preservative. 1 vial / 75 μL.

5. Melatonin Assay Diluent
Contains: phosphate buffer, sodium chloride, protein, stabilizer and preservative. 1 bottle / 30 mL

6. Wash Buffer Concentrate (10X) Dilute before use according to Reagent Preparation.
Contains: phosphate buffer, detergent, preservative.

7. TMB Substrate Solution
Non-toxic, ready to use. 1 bottle / 25 mL.

8. Stop Solution 1 bottle / 12.5 mL.

9. Adhesive Plate Covers 2

Storage

All unopened components of this kit are stable at 2-8°C until the kit's expiration date.

Performance Characteristics

Spike and Recovery
Two saliva samples containing different levels of endogenous Melatonin were spiked with known quantities of Melatonin and assayed.

Sample Dilution Recovery
Three saliva samples containing different levels of endogenous Melatonin were diluted in assay diluent and assayed.

Precision

The intra-assay precision was determined from the mean of 19-20 replicates each.

The inter-assay precision was determined from the mean of average duplicates for 10 separate runs.

Sensitivity

Analytical Sensitivity
The lower limit of sensitivity was determined by interpolating the mean optical density minus 2 SDs of 30 sets of duplicates at the 0 pg/mL level. The minimal concentration of Melatonin that can be distinguished from zero is 1.35 pg/mL.

Functional Sensitivity
The functional sensitivity was determined by assaying 60 saliva samples at a concentration level resulting in a CV of approximately 20%. The functional sensitivity of the salivary Melatonin EIA Kit is 3.31 pg/mL.

General Description

Melatonin (N-acetyl-5-methoxytryptamine) is a compound secreted mainly by the pineal gland, but, synthesized also in many other tissues and cells. In humans, nocturnally peaking oscillations of Melatonin are involved in sleep-wakefulness where Melatonin concentrations are lower during the day. In recent years, the role of Melatonin and its metabolites have been identified as potent, broad acting antioxidants and free radical scavengers in addition to playing a role in the upregulation of antioxidant enzymes. Melatonin levels in plasma are paralleled by corresponding variations in saliva where the saliva concentrations are about 30% of that found in plasma. Measurement of salivary Melatonin is advantageous, especially to avoid invasive venipuncture procedures.

Standard Curve

The results shown below are for illustration only and should not be used to calculate results from another assay.

General Notes

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Levomepromazine and clozapine induce the main human cytochrome P450 drug metabolizing enzyme CYP3A4

PHARMACOLOGICAL REPORTS

Authors: Danek, Przemyslaw J.; Basinska-Ziobron, Agnieszka; Wojcikowski, Jacek; Daniel, Wladyslawa A.

Abstract

Background Cytochrome P450 (CYP) enzymes are involved in the metabolism of many important endogenous substrates (steroids, melatonin), drugs and toxic xenobiotics. Their induction accelerates drug metabolism and elimination. The present study aimed at examining the inducing abilities of two antipsychotic drugs levomepromazine and clozapine for the main CYPs. Methods The experiments were performed using cryopreserved human hepatocytes. The hepatotoxicity of levomepromazine and clozapine was assessed after exposure to the neuroleptics (LDH test). CYP activities were measured in the incubation medium using the CYP-specific reactions: caffeine 3-N-demethylation (CYP1A1/2), diclofenac 4 '-hydroxylation (CYP2C9), perazineN-demethylation (CYP2C19) and testosterone 6 beta-hydroxylation (CYP3A4). In parallel,CYPmRNA levels were measured in neuroleptic-treated hepatocytes. Results The results indicate that levomepromazine and clozapine induce the expression of main CYP enzyme CYP3A4 in human hepatocytes. Levomepromazine and clozapine at concentrations of 2.5 and 10 mu M, respectively, caused a significant increase in the mRNA level and activity of CYP3A4. Both neuroleptics did not produce any changes in CYP1A1/2, CYP2C9 and CYP2C19. Conclusion Levomepromazine and clozapine induce CYP3A4 in human hepatocytes in vitro. Further in vivo studies are advisable to confirm the CYP3A4 induction by levomepromazine and clozapine in the liver, and to assess the effect of these drugs on their own metabolism and on the biotransformation of other co-administered drugs which are the CYP3A4 substrates.

Exercise and caloric restriction improve cardiovascular and erectile function in rats with metabolic syndrome

INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH

Authors: Cevikelli-Yakut, Zatiye Ayca; Ozcelik, Reyhan; Cevik, Ozge; Sener, Tarik Emre; Sener, Goksel

Abstract

The aim of this study is to examine the possible benefits of exercise and caloric restriction (CR) on cardiovascular hemodynamics, erectile function, and antioxidant system in metabolic syndrome (MS). Sixty male Spraque-Dawley rats were divided into five groups; control, MS, MS + CR, MS + exercise (EXC), and MS + CR + EXC. To induce MS, 10% fructose solution was applied for 3 months. Thereafter, in CR groups calorie was restricted 40% and in EXC groups swimming was performed for 6 weeks. Body weight, blood glucose, and blood pressure (BP) levels were measured before and after MS induction and at the end of the experiment. After decapitation, tumor necrosis factor (TNF)-alpha, adiponectin (ADP), and plasminogen activator inhibitor (PAI)-1 levels were investigated in blood, oxidative stress parameters were examined in heart, aorta, and corpus cavernosum (CC) tissues. Isometric contraction in isolated tissue bath was studied in aorta and CC tissues. Animals subjected to exercise and CR had decreased BP and blood glucose levels. Impaired contraction-relaxation responses in MS group were improved with exercise and CR. MS-induced increase in TNF-alpha, PAI-1, malondialdehyde (MDA), and decrease in ADP, glutathione (GSH), and superoxide dismutase (SOD) were normalized with exercise and CR. Exercise and CR may be beneficial against changes in cardiovascular hemodynamics caused by MS.