Salivary Melatonin ELISA Kit (DEIA-S10024)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Intended Use
The Salimetrics Salivary Melatonin kit is a competitive immunoassay specifically designed and validated for the quantitative measurement of salivary melatonin. It is not intended for diagnostic use. It is intended only for research use in humans and some animals. Salimetrics has not validated this kit for serum or plasma samples.
Please read the complete kit insert before performing this assay. Failure to follow kit procedure and recommendations for saliva collection and sample handling may result in false values.
For further information about this kit, its application, or the procedures in this insert, please contact the technical service team at Salimetrics or your local sales representative.
All components of this kit are stable at 2 - 8°C until the kit's expiration date.
The intra-assay precision was determined from the mean of 20 replicates each sample.
The inter-assay precision was determined from the average of 20 replicates across 10 runs, replicates of 2 per run.
Analytical Sensitivity: The lower limit of sensitivity was determined by interpolating the mean optical density minus 2 SDs of 10 sets of duplicates randomized across the plate within one run at the 0 pg/mL level. The minimal concentration of Melatonin that can be distinguished from 0 is 0.58 pg/mL.
Functional Sensitivity: The functional sensitivity was determined as the concentration of 11 saliva samples, tested as 20 replicates each, resulting in a CV of 30% or less. The functional sensitivity of the Salivary Melatonin ELSIA is 1.9 pg/mL.
General Description
Melatonin (N-acetyl-5-methoxytryptamine) is a compound secreted mainly by the pineal gland, but synthesized also in many other tissues and cells. In humans, nocturnally peaking oscillations of melatonin are involved in sleep-wakefulness where melatonin concentrations are lower during the day. In recent years, the role of melatonin and its metabolites have been identified as potent, broad acting antioxidants and free radical scavengers in addition to playing a role in the upregulation of antioxidant enzymes. Melatonin levels in plasma are paralleled by corresponding variations in saliva where the saliva concentrations are about 30% of that found in plasma. Measurement of salivary melatonin is advantageous, especially to avoid invasive venipuncture procedures.


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