Mouse Mast Cell Tryptase(MCT) ELISA Kit

Sample

serum, plasma, cell culture supernates and tissue homogenates

Species Reactivity

Mouse

Detection Method

sELISA

Intended Use

For the quantitative determination of mouse mast cell tryptase (MCT) concentrations in serum, plasma, cell culture supernates and tissue homogenates.

Contents of Kit

1. Assay plate (12 × 8 coated Microwells), 1(96 wells)
2. Standard (Freeze dried), 2
3. Biotin-antibody (100× concentrate), 1 × 120 μl
4. HRP-avidin (100× concentrate), 1 × 120 μl
5. Biotin-antibody Diluent, 1 × 15 ml
6. HRP-avidin Diluent, 1 × 15 ml
7. Sample Diluent, 1 × 50 ml
8. Wash Buffer (25× concentrate), 1 × 20 ml
9. TMB Substrate, 1 × 10 ml
10. Stop Solution, 1 × 10 ml
11. Adhesive Strip (For 96 wells), 4
12. Instruction manual, 1

Storage

Unopened kit: Store at 2 - 8°C. Do not use the kit beyond the expiration date.
Opened kit:
Coated assay plate: May be stored for up to 1 month at 2 - 8°C.Try to keep it in a sealed aluminum foil bag,and avoid the damp.
Standard/Biotin-antibody/HRP-avidin: May be stored for up to 1 month at 2 - 8° C.If don't make recent use, better keep itstore at -20°C.
Biotin-antibody Diluent/HRP-avidin Diluent/Sample Diluent/Wash Buffer/TMB Substrate/Stop Solution: May be stored for up to 1 month at 2 - 8°C.

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Detection Range

31.25 pg/ml-2000 pg/ml.

Sensitivity

The minimum detectable dose of mouse MCT is typically less than 15.625 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.