Characterization of Beauveria bassiana isolates from Kyrgyzstan
JOURNAL OF INVERTEBRATE PATHOLOGY
Authors: Doolotkeldieva, Tinatin; Bobusheva, Saikal; Kulmanbetova, Aijamal; Zholdoshbekova, Sezim; Kyzy, Aygerim Amanbek
Abstract
We report on the enzootic foci of the insect pathogenic fungus, Beauveria bassiana, found in high meadows in the middle mountain steppes of Kyrgyzstan, at elevations from 1000 m to 2200 m. The growth characteristics of various B. bassiana isolates on different media and as a function of temperature were studied. In addition, the ability of the fungal isolates to produce enzymes with amylase, protease and lipase activities was investigated. Dense biomass production on inexpensive solid media (oatmeal and bean oil meal) produced conidia used for insect bioassays targeting white grub larvae (Phyllophaga fullo, Coleoptera, Scarabaeidae) and nymphal and adult populations of whiteflies (Trialeurodes vaporariorum, Hemiptera, Aleyrodidae). The efficacies of the tested B. bassiana strains for third instar white grub larvae varied, with only two strains showing high entomopathogenic activity. At 25 degrees C, mortality reached 73% for Bay.5-Gal and 74% for Bay.1-Lep at 55 d post-infection, but was lower, 27% and 29%, respectively, at 12 degrees C. These two strains produced significantly higher mortality in adult and whitefly nymphs, with 65-75% mortality 6 d post-infection. Based on morphological characters, including production of ellipsoidal conidia, and molecular characters (ITS, partial 18S (SSU rDNA) and EF1-alpha sequences), the isolates were identified as Beauveria bassiana belonging to Clade E from Asia. Our results add to data on the diversity of ecosystems inhabited by B. bassiana and provide a local resource for pest control efforts.
The MESAS Project: ALMA Observations of the F-type Stars gamma Lep, gamma Vir A, and gamma Vir B
ASTROPHYSICAL JOURNAL
Authors: White, Jacob Aaron; Tapia-Vazquez, F.; Hughes, A. G.; Moor, A.; Matthews, B.; Wilner, D.; Aufdenberg, J.; Hughes, A. M.; De la Luz, V; Boley, A.
Abstract
The spectrum of stars in the submillimeter to centimeter wavelength range remains poorly constrained due to a lack of data for most spectral types. An accurate characterization of stellar emission in this regime is needed to test stellar atmosphere models, and is also essential for revealing emission associated with unresolved circumstellar debris. We present Atacama Large Millimeter/submillimeter Array observations of the three nearby, main-sequence, debris-poor, F-type stars gamma Lep, gamma Vir A, and gamma Vir B at 0.87 and 1.29 mm. We use these data to constrain semiempirical atmospheric models. We discuss the atmospheric structure of these stars, explore potential short-term variability, and the potential impact on debris disk studies. These results are part of an ongoing campaign to obtain long wavelength observations of debris-poor stars, entitled Measuring the Emission of Stellar Atmospheres at Submillimeter/millimeter wavelengths.