Human LEP peptide

Name: Human LEP peptide
SKU: DAG332
Rating: 5 (1 reviews)

Recombinant Human LEP peptide

COA

SDS

Datasheet

Summary

Product Overview

RecombinantHuman Leptin is a single non-glycosylated polypeptide chain containing 146amino acids and having a molecular weight of 16 kDa. The sequence of thefirst five N-terminal amino acids was determined to beAla-Val-Pro-Ile-Gln.

Nature

Recombinant

Tag/Conjugate

Unconjugated

Alternative Names

OB; OBS

Format

Purified, Lyophilized; Reconstitute in water. Further dilutions can be made in otheraqueou buffers.

Concentration

1 mg/ml (OD280nm, E0.1% = 0.878) (prior tolyophilization)

Buffer

Lyophilized from 0.0045mM NaHCO3

Preservative

None

Storage

2-8°C short term, -20°C long term

Introduction

Leptin, the "satiety hormone", is a hormone made by fat cells which regulates the amount of fat stored in the body. It does this by adjusting both the sensation of hunger, and adjusting energy expenditures. Hunger is inhibited (satiety) when the amount of fat stored reaches a certain level. Leptin is then secreted and circulates through the body, eventually activating leptin receptors in the arcuate nucleus of the hypothalamus. Energy expenditure is increased both by the signal to the brain, and directly via leptin receptors on peripheral targets. The effect of leptin is opposite to that of ghrelin, the "hunger hormone". Ghrelin receptors are on the same brain cells as leptin receptors, so these cells receive competing satiety and hunger signals. Leptin and ghrelin, along with many other hormones, participate in the complex process of energy homeostasis.

Entrez Gene ID

3952

Antigen Description

Leptin (Greek leptosmeaning thin) is a 16 kDa protein hormone that plays a key role in regulatingenergy intake and energy expenditure, including appetite and metabolism. Itis one of the most important adipose derived hormones. The Ob(Lep) gene (Obfor obes

Keywords

LEP; leptin; leptin (murine obesity homolog) , leptin (obesity homolog, mouse) , OB, OBS; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse); OB; OBS; FLJ94114;

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Association ofLEPRpolymorphisms with egg production and growth performance in female Japanese quails

ANIMAL BIOTECHNOLOGY

Authors: El-Tarabany, Mahmoud S.; Saleh, Ayman A.; El-Araby, Iman E.; El-Magd, Mohammed A.

Abstract

This study aimed to screen intron 8 of the leptin receptor (LEPR) gene for polymorphisms in female Japanese quails. Two adjacent novel SNPs (A277G and A304G) were detected using PCR-SSCP and sequencing. These SNPs produced three haplotypes (AA/AA, AG/AG, and GG/GG) that were significantly (p <= 0.05) associated with growth and egg production traits. GG/GG haplotype-quails had significantly (p <= 0.05) lower egg production, feed intake, growth performance, lipid profile, serum levels of sex hormones (estradiol, progesterone, FSH, LH), and ovarian expressions of survivin,FSHR, andIGF1than other quails. However, GG/GG quails had significantly (p <= 0.05) higher serum levels of LEP and mRNA levels of LEPR, LEP, and caspase 3 in the hypothalamus and ovaries. These higher levels of LEP/LEPRcould not only reduce feed intake and body weight gain but also could induce apoptosis of ovarian cells (as indicated by lower survivin andIGF1and higher caspase3 expression) which could inhibit the development of the follicles and the release of sex hormones with a subsequent decrease in egg production in GG/GG quails. Therefore, with these results, we suggest selecting Japanese quails with AA/AA and AG/AG haplotypes to improve the reproduction and growth performance of this flock.

THE PENITENTIARY SYSTEM AS A SOCIO-OCCUPATIONAL SPACE FOR SOCIAL ASSISTANT

HUMANIDADES & INOVACAO

Authors: Cavalcante Carvalho, Anna Karoline

Abstract

This article proposes to characterize the penitentiary system as a socio-occupational space for the Social Service professional, for that, a brief history of the Prison System and penalties will be made for a better delimitation of this universe. Expanding the understanding about the prison, we will situate it within the capitalist society, pointing out its intrinsic relationship with the field of law, and how this is put to the Social Worker. Finally, we will make inference to some aspects related to the profile of the prison population, who present themselves as users of the social assistance services provided for in the Law of Penal Executions - LEP.