Anti-LEP polyclonal antibody

A consistent finding in migraine is reduced cortical habituation to repetitive sensory stimuli. This study investigated brain dynamics underlying the atypical habituation to painful stimuli in interictal migraine. We investigated modulations in effective connectivity between the sources of laser evoked potentials (LEPs) from a first to final block of trigeminal LEPs using dynamic causal modelling (DCM) in a group of 23 migraine patients and 20 controls. Additionally, we looked whether the strength of dynamical connections in the migrainous brain is initially different. The examined network consisted of the secondary somatosensory areas (lS2, rS2), insulae (lIns, rIns), anterior cingulate cortex (ACC), contralateral primary somatosensory cortex (lS1), and a hidden source assumed to represent the thalamus. Results suggest that migraine patients show initially heightened communication between lS1 and the thalamus, in both directions. After repetitive stimulations, connection strengths from the thalamus to all somatosensory areas habituated in controls whereas this was not apparent in migraine. Together with further abnormalities in initial connectivity strengths and modulations between the thalamus and the insulae, these results are in line with altered thalamo-cortical network dynamics in migraine. Group differences in connectivity from and to the insulae including interhemispheric connections, suggests an important role of the insulae.

Even though meliponines are among the greatest pollinating agents in Neotropical ecosystems, the populations of these bees are in decline due to anthropic actions, such as the indiscriminate use of pesticides. Neonicotinoids, such as Imidacloprid, are used in many crops and have been shown to be one of the pesticides groups with the most damaging effects on the health of bee colonies. The goals of this work were (1) to evaluate the ability of Melipona quadrifasciata, a native Brazilian stingless bee species, to detect the presence of Imidacloprid in artificial diets, and (2) to evaluate how this insecticide may affect their behavior. For this purpose, behavioral experiments were carried out in observation arenas where artificial flowers containing different concentrations of Imidacloprid (0%, 1%, 5%, 10%, and 20%) were made available to forager bees and their choice and behavior were registered. Our results showed that bee choices were not affected by the different concentrations of Imidacloprid, even at extremely high doses in the flowers (20%). It was also found that the presence of this insecticide interfered with the communication between individuals as a result of reduced antennation events. Both findings, the apparent inability of bees to detect the presence of the insecticide and the change in behavior caused by the exposition to the volatilized product, may rapidly lead to colony losses in nature.