Laminin-Ab ELISA Kit

Sample

serum

Species Reactivity

Human

Intended Use

Enzyme immunoassay for the quantitative and qualitative determination of IgG antibodies against laminin-1 in human serum.

Contents of Kit

5x Sample Buffer: 1 x20 ml
50x Wash Buffer: 1 x20 ml
Negative Contro: l 1x1.5 ml
Positive Control: 1 x1.5 ml
Cut-off Calibrator: 1 x1.5 ml
Calibrators: 6x1.5 ml
Conjugate: 1 x15 ml
TMB Substrate: 1 x15 ml
Stop Solution: 1x 15 ml
Microtiterplate: 12x8 well

Storage

2-8°C

Precision

Detection Range

3 x 300 U/ml; cut-off 15 U/ml

Sensitivity

1.0 U/ml

General Description

Laminin-Ab ELISA is a solid phase enzyme immunoassay employing highly purified native human laminin-1 for the separate quantitative and qualitative detection of IgG antibodies against laminin-1 in human serum. Antibodies against laminin-1 can be found in patients with recurrent abortion and infertility associated with endometriosis. Laminins, multifunctional glycoproteins of the basement membrane, are involved in diverse biological activities, including the promotion of cell adhesion, migration, proliferation and differentiation, as well as the formation of the scaffolding network in basement membranes. To date, at least 15 different isoforms of laminin have been identified and are known to display tissue-specific expression during different stages of development. Laminin-1, composed of a1,b1 and g1 chains, is the earliest synthesized network-forming component during embryogenesis and plays an important role in embryonic development, embryonic implantation and placentation. In blastocytes or early implanting mouse embryo, laminin-1 is localized in the inner cell mass and trophectoderm basement membrane. As implantation proceeds, laminin-1 is expressed in chorionic basement membrane and in Reithert's membrane near ectoplacental cone. Recent human studies indicate that IgG autoantibodies againist laminin-1 are significantly associated with recurrent first-trimester miscarrages. In animal models, active immunization with mouse laminin-1 caused abortions in anti-laminin-1 positive mice. It was suggested that anti-laminin-1 antibodies may have a harmful effect on events at early stages of pregnancy, such as embryonic implantation, embryogenesis, placental vascularization, and/or placental nutrient transport. Morover recent clinical studies also showed that IgG laminin-1 antibodies are significantly associated with endometriosis in infertile patients. Thus, measurement of IgG anti-Laminin-1 autoantibodies may be a useful tool for diagnosing reproductive failure, such as recurrent abortion and infertility associated with endometriosis.

Standard Curve