Human Laminin 521 ELISA Kit

Sample

Serum and cell culture supernates

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

The kit is developed for the detection and quantitative determination of Laminin 521 in human serum and cell culture supernates. It is intended for research use only (RUO).

Contents of Kit

1. Pre-coated Anti-Laminin 521 Antibody Microplate: 1 plate, store at 2-8°C once opened
2. Human Laminin 521 Standard (Lyophilized): 20 μg, store at -70°C once opened
3. Biotin-Anti-Laminin Antibody (Lyophilized): 20 μg, store at -70°C once opened
4. Streptavidin-HRP: 50 μL, store at 2-8°C (avoid light) once opened
5. 10× Washing Buffer: 50 mL, store at 2-8°C once opened
6. 2× Dilution Buffer: 50 mL, store at 2-8°C once opened
7. Substrate Solution: 12 mL, store at 2-8°C (avoid light) once opened
8. Stop Solution: 7 mL, store at 2-8°C once opened

Storage

1. Unopened kit should be stored at 2°C-8°C upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per Reagents And Materials Provided. The shelf life is 30 days from the date of opening.

Precision

Intra-assay Precision: Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision: Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

Sensitivity

The minimum detectable concentration of Laminin 521 is 9.170 pg/mL. The minimum detectable concentration was determined by adding twice standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

General Description

Human Laminin 521 Protein is a recombinant human protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs). Laminin 521 has been proven to maintain normal growth characteristics and stemness in multiple PSC lines without simultaneous differentiation, which includes ESC, iPSC, MSC, etc. In addition, Laminin 521 has been demonstrated to support PSC growth for >10 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. To support the development of cell therapy drugs, Creative Diagnostics developed Human Laminin 521 ELISA Kit (Residue Testing) with rigorous methodological validation, which is used for the detection and quantitative determination of Laminin 521 in human serum and cell culture supernates. Besides, this kit can also be used for the quantitative determination of GMP human Laminin 521 Protein concentrations.

Standard Curve