Lactoferrin ELISA Kit

Name: Lactoferrin ELISA Kit
SKU: DEIA053J
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Stool

Species Reactivity

Human

Detection Method

Sandwich-ELISA

Intended Use

Lactoferrin ELISA kit is an enzyme immunoassay intended for the determination of lactoferrin in stool. For research use only.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Pre-coated microtiter plate	12 × 8 wells	2-8°C
2	Wash buffer concentrate (10×)	2 × 100 mL	2-8°C
3	Extraction buffer concentrate (2.5×)	1 × 100 mL	2-8°C
4	Conjugate concentrate	1 × 100 μL	2-8°C
5	Standards (lyophilised)	4 × 5 vials	2-8°C
6	Control (lyophilised)	4 × 1 vial	2-8°C
7	Control (lyophilised)	4 × 1 vial	2-8°C
8	Substrate	1 × 15 mL	2-8°C
9	Stop solution	1 × 15 mL	2-8°C
10	Sample dilution buffer	1 × 50 mL	2-8°C

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date.

Precision

Repeatability (Intra-Assay); n = 40
The repeatability was assessed with 2 stool samples under constant parameters (same operator, measurement system, day and kit lot).

Reproducibility (Inter-Assay); n = 16
The reproducibility was assessed with 2 stool samples under varying parameters (different operators, measurement systems, days and kit lots).

Sensitivity

The following values have been estimated based on the concentrations of the standards without considering possibly used sample dilution factors.
Limit of blank, LoB: 0.222 ng/ml
Limit of detection, LoD: 0.416 ng/ml
Limit of quantitation, LoQ*: 0.416 ng/ml
*The evaluation was performed according to the CLSI guideline EP17-A2. The specified accuracy goal for the LoQ was 20 % CV.

General Description

Lactoferrin is a 76 kDa iron-binding glycoprotein which is synthesised and stored in the secondary granules of neutrophils. It is also present in several secretory fluids, such as milk, saliva, tears, and nasal secretions.
Lactoferrin can exist in different polymeric forms ranging from monomers to tetra mers; it tends to polymerise especially at high concentrations.
The physiological activities of lactoferrin include regulation of iron homeostasis, innate defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis.
During intestinal inflammation, polymorphonuclear neutrophils infiltrate the mucosa and release lactoferrin by degranulation, which results in an increased excretion of lactoferrin into the faeces. Faecal lactoferrin is therefore a marker for neutrophilic intestinal inflammation.

Citations