Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Rat LTF/LF (Lactoferrin) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
31.2-2000 pg/mL
Citations
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Fine, DH; Toruner, GA; et al. A Lactotransferrin Single Nucleotide Polymorphism Demonstrates Biological Activity That Can Reduce Susceptibility to Caries. INFECTION AND IMMUNITY 81:1596-1605(2013).
Corvazier, E; Bredoux, R; et al. Expression of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA) 3 proteins in two major conformational states in native human cell membranes. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1788:587-599(2009).
COA
Certificate of Analysis
