IRAK1 (Phospho-Thr209) ELISA Kit

Sample

Cell culture

Detection Method

iELISA

Intended Use

The Fluorometric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines.

Contents of Kit

1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10× TBS: 24 mL
3. Quenching Buffer: 24 mL
4. Blocking Buffer: 50 mL
5. 15× Wash Buffer: 50 mL
6. 100× Anti-IRAK1 (Phospho-Thr209) Antibody (Rabbit Polyclonal): 60 μL
7. 100× Anti-IRAK1 Antibody (Rabbit Polyclonal): 60 μL
8. 100× Anti-GAPDH Antibody (Mouse Monoclonal): 110 μL
9. Dye1-Conjugated Anti-Rabbit IgG Antibody: 12 mL
10. Dye2-Conjugated Anti-Mouse IgG Antibody: 12 mL
11. Primary Antibody Diluent: 12 mL
12. Adhesive Plate Seals: 2 seals

Storage

Store entire kit at 4°C until use.
Kit expiration is 6 months from date of shipment

General Description

Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1°) antibodies while Dye-conjugated and Dye-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the dye conjugated to the 2° antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method.
A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. If a phosphorylated target is being detected, an antibody against the nonphosphorylated counterpart will be provided for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

General Notes

Phosphorylation ELISA Kits