Anti-IRAK1 monoclonal antibody, clone 4G8 Knockout Validated

Antibody Isotype

IgG2a

Clone

4G8

Species Reactivity

Human

Immunogen

Recombinant fragment, corresponding to amino acids 530-693 of Human IRAK with a proprietary tag (AAH54000).

Conjugate

Application Notes

WB: 1/500 - 1/1000; IHC-P: 5 μg/ml; ICC/IF: 10 μg/ml;

Alternative Names

IRAK1; interleukin-1 receptor-associated kinase 1; IRAK; pelle; IRAK-1; Pelle homolog;

Entrez Gene ID

UniProt ID

Antigen Description

Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.

Pathway

Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; IL-1 Signaling Pathway, organism-specific biosystem;