Sample
Serum, plasma, tissue homogenates
Species Reactivity
Canine
Intended Use
For the quantitative determination of endogenic canine inhibin beta-B (Inhbb) concentrations in serum, plasma, tissue homogenates.
Contents of Kit
1. Assay plate: 1 (96 wells)
2. Standard: 6 × 0.5 ml (0, 5, 20, 80, 250, 1000 pg/ml)
3. Antibody: 1 × 6 ml
4. HRP-conjugate: 1 × 6 ml
5. Wash Buffer (20 × concentrate): 1 × 15 ml
6. Substrate A: 1 × 7 ml
7. Substrate B: 1 × 7 ml
8. Stop Solution: 1 × 7 ml
9. Adhesive Strip (For 96 wells): 4
10. Instruction manual: 1
Storage
Unopened kit: Store at 2 - 8°C. Do not use the kit beyond the expiration date.
Opened kit: May be stored for up to 1 month at 2 - 8°C.
*Provided this is within the expiration date of the kit.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Detection Range
5 pg/ml-1000 pg/ml.
Sensitivity
The minimum detectable dose of canine Inhbb is typically less than 2.5 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest canine Inhbb concentration that could be differentiated from zero.
Citations
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COA
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