Influenza A Virus Nucleoprotein Antigen ELISA Kit

Sample

Complex sample matrices(human and veterinary sources)

Detection Method

sELISA

Intended Use

Influenza A Virus Nucleoprotein Antigen ELISA Kit is a qualitative procedure for the detection of Influenza A nucleoprotein antigen in complex sample matrices derived from both human and veterinary sources.

Contents of Kit

1. IAV Antigen Capture Plate (96 tests)-2 ea.
2. Sample Preparation Reagent (1x)-12 ml
3. IAV Positive Control (1x)-1 ml
4. IAV Negative Control (1x)- 2 x 1.5 ml
5. Wash Buffer (20x)-30 ml
6. IAV Detection Antibody, HRP-labeled (1x)-22 ml
7. Chromagen Solution (1x)-22 ml
8. Stop Solution (1x)-22 ml
9. Sample Dilution Tray-2 ea.

Storage

Store all kit components at 2-8°C. Crystal formation may occur in the wash buffer concentrate during prolonged storage at 2-8°C.The crystals can be re-dissolved by swirling the bottle in warm tap water.

General Description

Influenza viruses can be divided into three classes, A, B, and C, largely based upon conserved antigenic differences in the internal nucleoprotein. Influenza A virus, typically encountered more frequently than types B and C, and associated with the majority of serious epidemics, can be further subdivided into strains or subtypes based on antigenic differences in the external hemagglutinin proteins (H1-H16) and neuraminidase proteins (N1-N9).
A variety of wild waterfowl appear to be the predominant natural reservoir for Influenza A viruses and subtypes representing most of the hemagglutinin and neuraminidase combinations can be found circulating in these birds. Historically, human influenza virus infections have been associated with H1N1, H2N2, and H3N2 subtypes of influenza A, although a recent (1997) and significant outbreak in Hong Kong was identified as an H5N1 subtype. This outbreak was not only significant because it resulted in 18 human infections and 6 deaths, but it also represented the first known demonstration of avian influenza virus transmission to humans.
While influenza A subtype identification is extremely important (vaccine production, epidemiology), the rapid and accurate differentiation of influenza A from influenza B and C and other respiratory agents in humans and animals is also important (treatment and biosecurity).
The Influenza A nucleoprotein antigen ELISA kit is a capture ELISA for the qualitative measurement of influenza A virus nucleoprotein antigen and can be completed in less than 1.5 h and contains only one wash step. In addition, the test kit incorporates proprietary diluents that are designed to prevent the development of nonspecific signal derived from complex sample matrix effects and/or the nonspecific adsorption of reactive test components which result in improvements in both sensitivity and specificity.