Influenza A IgM ELISA Kit

Name: Influenza A IgM ELISA Kit
SKU: DEIA355
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Sample

Serum, Plasma and Cerebrospinal Fluid.

Species Reactivity

Human

Detection Method

ELISA

Intended Use

The Influenza A IgM tests are quantitative and qualitative immunoassays for the detection of human antibodies in serum or plasma directed against conserved nucleo- and matrix proteins of Influenza A Viruses. The Influenza A IgM tests are recommended for the sensitive detection of acute infections, particularly in children.

Contents of Kit

1.ELISA Microplate: 12 × 8
Break apart microtiter test strips each with eight antigen coated single wells.
The coating material is inactivated.
2.Standard Serum (ready-to-use) 2 × 2 ml
Human serum in protein containing phosphate buffer; negative for anti-HIV Ab, HBsAg (Hepatitis B-Virus surface antigen) and anti-HCV Ab;
preservative: < 0.1 % sodium azide; colouring: Amaranth O.
3.Negative Control Serum (ready-to-use) 2 ml
Human serum in protein containing phosphate buffer; negative for anti-HIV Ab, HBsAg (Hepatitis B-Virus surface antigen) and anti-HCV Ab;
preservative: < 0.1 % sodium azide; colouring: Lissamin Green V.
4.Anti-Human IgG Conjugate (ready-to-use) 13 ml
Anti-human IgG polyclonal antibody, conjugated to alkaline phosphatase, stabilised with protein stabilisation solution;
preservative: < 0.1 % methylisothiazolone, < 0.1 % bromnitrodioxane.
5.Washing Solution Concentrate (sufficient for 1000 ml) 33.3 ml
Sodium chloride solution with Tween 20 and 30 mM Tris/HCl, pH 7.4;
preservative: < 0.1 % sodium azide.
6.Dilution Buffer (ready-to-use) 2 × 50 ml
Protein containing phosphate buffer with Tween 20;
preservative: < 0.1 % sodium azide; colouring: 0.01 g/l Bromphenol blue.
7.Stopping Solution (ready-to-use) 15 ml
< 0.1 N sodium hydroxide, 40 mM EDTA.
8.Substrate (ready-to-use) 13 ml
Para-nitrophenylphosphate in solvent free buffer;
preservative: < 0.1 % sodium azide.

Storage

The kit is stored at 2 - 8°C (Avoid Direct Light), and not be frozen or thawed. The product is valid for 12 months. After opening, store at 2 - 8°C, it can be stable for 6 months, avoid contamination.

Performance Characteristics

The Influenza A Virus IgM test was validated by the analysis of 105 serum samples from healthy blood donors and 76 samples from patients with suspected Influenza Virus infection. The ELISA of a European manufacturer was used as a reference. Sera classified as borderline were not included in the calculation.
Sensitivity: > 95.2 %
Specificity: > 98.5 %
Reproducibility:

Detection Range

The borderline ranges of the Influenza A Virus IgM tests are specified on the quality control certificates and indicate the range of borderline test results. Values below this range indicate a negative test result; values above the borderline range are interpreted positive.

Citations

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Datasheet

Certificate of Analysis

Safety Data Sheet

Infectious Disease Test Kits and Positive Controls

Customer Reviews

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Zinc Finger CCCH-Type Antiviral Protein 1 Restricts the Viral Replication by Positively Regulating Type I Interferon Response

FRONTIERS IN MICROBIOLOGY

Authors: Zhang, Baoge; Goraya, Mohsan Ullah; Chen, Na; Xu, Lifeng; Hong, Yan; Zhu, Meiyi; Chen, Ji-Long

Abstract

Zinc finger CCCH-type antiviral protein 1 (ZC3HAV1) is a host antiviral factor that can repress translation and promote degradation of specific viral mRNAs. In this study, we found that expression of ZC3HAV1 was significantly induced by infection with influenza A virus (IAV) and Sendai virus (Sev). It was shown that deficiency of IFNAR resulted in a dramatic decrease in the virus-induced expression of ZC3HAV1. Furthermore, transfection with poly(I:C) and treatment with interferon beta (IFN-beta) induced the ZC3HAV1 expression. Interference with the endogenous expression of ZC3HAV1 enhanced the replication of influenza virus by impairing the production of IFN-beta and MxA, following the infection of influenza virus. In contrast, ectopic expression of ZC3HAV1 significantly restricted the replication of influenza virus by increasing the IFN-beta expression. In addition, ZC3HAV1 also promoted the induction of tumor necrosis factor and interleukin 6. These results suggest that ZC3HAV1 is induced by IFN-beta/IFNAR signaling during IAV and Sev infection and involved in positive regulation of IFN-dependent innate antiviral response.

The Impact of TCR Signal Strength on Resident Memory T Cell Formation during Influenza Virus Infection

JOURNAL OF IMMUNOLOGY

Authors: Fiege, Jessica K.; Stone, Ian A.; Fay, Elizabeth J.; Markman, Matthew W.; Wijeyesinghe, Sathi; Macchietto, Marissa G.; Shen, Steven; Masopust, David; Langlois, Ryan A.

Abstract

Resident memory T cells (T-RM) in the lung are vital for heterologous protection against influenza A virus (IAY). Environmental factors are necessary to establish lung T-RM; however, the role of T cell-intrinsic factors like TCR signal strength have not been elucidated. In this study, we investigated the impact of TCR signal strength on the generation and maintenance of lung T-RM after IAV infection. We inserted high- and low-affinity OT-I epitopes into IAV and infected mice after transfer of OT-I T cells. We uncovered a bias in T-RM formation in the lung elicited by lower affinity TCR stimulation. TCR affinity did not impact the overall phenotype or long-term maintenance of lung T-RM. Overall, these findings demonstrate that T-RM formation is negatively correlated with increased TCR signal strength. Lower affinity cells may have an advantage in forming T-RM to ensure diversity in the Ag-specific repertoire in tissues.