Human Influenza A IgA ELISA Kit

Name: Human Influenza A IgA ELISA Kit
SKU: DEIA2368
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The ELISA test kit provides a semiquantitative in vitro assay for human antibodies of the IgA class against Influenza A virus in serum or plasma.

Contents of Kit

1. Microplate wells, coated with antigens, 12 microplate strips each containing 8 individual break-off wells in a frame, ready for use --- 12 x 8 .STRIPS.
2. Calibrator (IgA, human), ready for use, dark red, 1 x 2.0 ml .CAL.
3. Positive control (IgA, human), ready for use, blue, 1 x 2.0 ml .POS CONTROL.
4. Negative control (IgA, human), ready for use, green 1 x 2.0 ml .NEG CONTROL.
5. Enzyme conjugate, peroxidase-labelled anti-human IgA (rabbit), ready for use, orange, 1 x 12 ml .CONJUGATE.
6. Sample buffer, ready for use, light blue, 1 x 100 ml .SAMPLE BUFFER.
7. Wash buffer, 10x concentrate, colourless, 1 x 100 ml .WASH BUFFER 10x.
8. Chromogen/substrate solution, TMB/H₂O₂, ready for use, colourless, 1 x 12 ml .SUBSTRATE.
9. Stop solution, 0.5 M sulphuric acid, ready for use, colourless, 1 x 12 ml .STOP SOLUTION.
10. Test instruction --- 1 booklet
11. Quality control certificate --- 1 protocol

Storage

The test kit has to be stored at a temperature between +2°C to +8°C. Do not freeze. Unopened, all test kit components are stable until the indicated expiry date.

Performance Characteristics

Calibration: As no international reference serum exists for antibodies against influenza A viruses, results are provided in the form of ratios which are a relative measure for the concentration of antibodies.
For every group of tests performed, the extinction values of the calibrator and the ratios of the positive and negative controls must lie within the limits stated for the relevant test kit lot. A quality control certificate containing these reference values is included. If the values specified for the controls are not achieved, the test results may be inaccurate and the test should be repeated.
The activity of the enzyme used is temperature-dependent and the extinction values may vary if a thermostat is not used. The higher the room temperature during substrate incubation, the greater will be the extinction values. Corresponding variations apply also to the incubation times. However, the calibrators are subject to the same influences, with the result that such variations will be largely compensated in the calculation of the result.
Antigen: The antigen source is provided by inactivated allantois fluid of chicken embryos infected with the "Texas" (H3N2) strain of Influenza A virus, „Singapore" (H1N1) strain of Influenza A virus and "California" (H1N1, Porcine Influenza).

Detection Limit

The lower detection limit is defined as the mean value of an analyte-free sample plus three times the standard deviation and is the smallest detectable antibody titer. The detection limit of the Anti-Influenza A Virus ELISA (IgA) is ratio 0.04.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Hyperactivation of the NLRP3 inflammasome protects mice against influenza A virus infection via IL-1 beta mediated neutrophil recruitment

CYTOKINE

Authors: Niu, Junling; Wu, Shuxian; Chen, Mingkuan; Xu, Ke; Guo, Qiuhong; Lu, Ailing; Zhao, Liping; Sun, Bing; Meng, Guangxun

Abstract

Host innate immune system is critical for combating invading microbes including Influenza A virus (IAV). As an important arm of the innate immunity, the NLRP3 inflammasome has been found essential for protecting host against IAV challenge, while the mechanism remained elusive. Here we found that mice carrying a gain-of-function mutation in the Nlrp3 gene (Nlrp3(R258W)) are strongly resistant to IAV infection. Upon H1N1 IAV infection, the Nlrp3(R258W) mice exhibited decreased weight loss, increased survival rate and attenuated lung damage compared with WT littermate controls. Mechanistically, the resistance of Nlrp3(R258W) mice to IAV infection was dependent on IL-1 beta-mediated neutrophil recruitment. Upon IAV infection, mice carrying the Nlrp3(R258W) mutation produced more IL-1 beta than WT mice in the lung, which enhanced neutrophil recruitment locally. The recruited neutrophils facilitated IAV clearance, so that the viral load in Nlrp3(R258W) mice was lower than that in control mice. Conversely, neutrophil depletion in Nlrp3(R258w) mice compromised IAV clearance. Taken together, our results demonstrate a previously undescribed mechanism by which hyperactivation of the NLRP3 Inflammasome protects mice from IAV infection through IL-1 beta mediated neutrophil recruitment, thus suggest that positively fine tuning the physiological function of NLRP3 inflammasome can be beneficial for a mammalian host against IAV challenge.

The effects of the Xijiao Dihuang decoction combined with Yinqiao powder on miRNA-mRNA profiles in mice infected with influenza a virus

BMC COMPLEMENTARY MEDICINE AND THERAPIES

Authors: Li, Ke; Chen, Xiaoming; Zhong, Jing; Ye, Hehe; Zhang, Shujing; Ge, Dongyu; Wang, Xudan; Wu, Ying

Abstract

Background MicroRNAs (miRNAs) play vital roles in acute inflammatory and antiviral responses during influenza A virus (IAV) infection. The Xijiao Dihuang decoction combined with Yinqiao powder (XDY) is applied to remedy viral pneumonia in China and its therapeutic efficacy in pneumonic mice challenged with IAV was demonstrated; however, the underlying mechanisms remain elusive. Thus, this study aimed to explore the miRNA-mRNA profiles in the lungs of IAV-infected mice and investigate the therapeutic mechanisms of XDY involving miRNAs and associated pathways. Methods We detected the cellular miRNA contents in the lungs of mice treated with XDY (23 g/kg/d) for A/FM/1/47 (H1N1) (FM1) infection at 4 days postinoculation (dpi) and 7 dpi. MiRNA and mRNA high-throughput sequencing analyses, and miRNA and mRNA qRT-PCR analyses were used to detect and verify the relevant miRNAs and mRNAs. Conjoint analysis, GO enrichment analysis, and KEGG database analysis were applied to identify the miRNA-mRNA regulatory relationships. Results The quantities of differentially expressed miRNAs and mRNAs were upregulated over time. The data showed that 104 miRNAs and 3485 mRNAs were differentially expressed after challenge with FM1 on day 4, while 191 miRNAs and 6126 mRNAs were differentially expressed on day 7. The GO enrichment analysis and KEGG database data showed that the differentially expressed miRNAs and mRNAs were mainly enriched in JNK activity, MAPK phosphatase activity, and the TLR, Jak-STAT and TNF signalling pathways after treatment of FM1 infection with XDY. Generally, the expression trends of differentially expressed miRNAs and mRNAs based on the qRT-PCR results exhibited good consistency with the results of the high-throughput sequencing analysis. Conclusions MiRNAs and mRNAs were differentially expressed during FM1 infection. The therapeutic mechanisms of XDY in FM1-infected mice, might be related to regulating antiviral immunity and ameliorating excessive inflammatory responses by modulating the expression of dysregulated miRNAs and mRNAs involved in the ERK/JNK-AP-1, and IFN-beta/STAT signalling pathways.