Influenza A Nucleoprotein Antibody Inhibition ELISA Kit

Sample

Serum(human and veterinary sources)

Detection Method

cELISA

Intended Use

A highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of influenza A NP-specific antibodies in serum from human and veterinary sources.

Contents of Kit

1. IAB Antigen Capture Plate (96 tests) - 2 ea.
2. IAB Antigen Diluent (1x) - 24 ml
3. IAB Antigen Concentrate (1x) - 4 ml
4. IAB Positive Serum Control (1x) - 0.1 ml
5. IAB Negative Serum Control (1x) - 0.1 ml
6. Wash Buffer (20x) - 1 x 50 ml
7. IAB Detection Antibody, HRP-labeled (1x) - 12 ml
8. Chromagen Solution (1x) - 22 ml
9. Stop Solution (1x) - 22 ml
10. Sample Dilution Tray - 5 ea.

Storage

Store all kit components at 2-8°C. Crystal formation may occur in the wash buffer concentrate during prolonged storage at 2-8°C. The crystals can be re-dissolved by swirling the bottle in warm tap water.

Sensitivity

Titration of anti-H9N2 and anti-H1N1 positive chicken sera. (NOTE: sample dilutions represent dilution of positive sera in pool of normal [negative] chicken serum.
Each dilution was then diluted 1:21 into IAB Sample Diluent for assay as described above).

General Description

The assay utilizes"inhibition of binding" technology, it may be used with serum from any species, and therefore does not require speciesspecific conjugates. The assay can be completed in less than 1.5 hr., contains only one wash step, and incorporates proprietary diluents that are designed to prevent the development of nonspecific signal derived from sample matrix and/or the nonspecific adsorption of reactive test components. The result is an assay that is both highly sensitive and specific.