Sample
cell culture supernatants, serum, EDTA plasma, citrate plasma, heparin plasma
Detection Method
Sandwich ELISA
Intended Use
The Human sTNF-R (60kDa) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of Human sTNF-R (60kDa).
Contents of Kit
1. 1 aluminum pouch with a Microwell Plate coated with monoclonal polyclonal antibody to human sTNF-R (60kDa)
2. 1 vial (200 μL) HRP-Conjugate anti-human sTNF-R (60kDa) monoclonal antibody
3. 2 vials (50 μL) human sTNF-R (60kDa) Standard, 10 ng/mL upon dilution
4. 1 vial Control high, lyophilized
5. 1 vial Control low, lyophilized
6. 1 vial (5 mL) Assay Buffer Concentrate 20X (PBS with 1% Tween 20 and 10% BSA)
7. 1 bottle (50 mL) Wash Buffer Concentrate 20X (PBS with 1% Tween 20)
8. 1 vial (15 mL) Substrate Solution (tetramethyl-benzidine)
9. 1 vial (15 mL) Stop Solution (1M Phosphoric acid)
10. 1 vial (0.4ml) Blue-Dye
11. 1 vial (0.4ml) Green-Dye
Storage
Store the reagents at 2-8°C until expiration date. For more detailed information, please download the following document on our website.
Citations
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Van Hauwermeiren, F; Armaka, M; et al. Safe TNF-based antitumor therapy following p55TNFR reduction in intestinal epithelium. JOURNAL OF CLINICAL INVESTIGATION 123:2590-2603(2013).
Todd, I; Radford, PM; et al. Mutant tumor necrosis factor receptor associated with tumor necrosis factor receptor-associated periodic syndrome is altered antigenically and is retained within patients' leukocytes. ARTHRITIS AND RHEUMATISM 56:2765-2773(2007).
COA
Certificate of Analysis
