Human TRAIL ELISA

Sample

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The human TRAIL ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TRAIL. The human TRAIL ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Contents of Kit

1 vial (70 μl) Biotin-Conjugate anti-human TRAIL monoclonal antibody
1 vial (150 μl) Streptavidin-HRP
2 vials human TRAIL Standard lyophilized, 2 ng/ml upon reconstitution
1 vial (50 ml) Sample Diluent
1 bottle (50 ml) Wash Buffer Concentrate 20× (PBS with 1% Tween 20)
1 vial (15 ml) Substrate Solution (tetramethyl-benzidine)
1 vial (15 ml) Stop Solution (1M Phosphoric acid)

Storage

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Detection Range

15.6-1000 pg/ml

Detection Limit

5.0 pg/ml

Sensitivity

The limit of detection of human TRAIL defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 5 pg/ml (mean of 6 independent assays).

General Description

TRAIL (TNF-related-apoptosis-inducing-ligand) was found to be a member of the TNF family which mediates cell death in a wide variety of malignant cell lines and primary tumor cells.
TRAIL induces two different signals, cell death mediated by caspases and gene induction. The cytotoxic ligand TRAIL interacts with five receptors, only two of which (TRAIL-R1 and R2) have a death domain.
TRAIL, which is ubiquitous expressed, exhibits very complex signalling and tumor-selective induction of apoptosis.
TRAIL is involved in lymphatic neoplastic disorders and thyroid diseases, and the treatment of cancer like melanoma.

Standard Curve