Human Cripto-1 ELISA Kit

Sample

serum, plasma, urine

Species Reactivity

Human

Detection Method

sandwich

Intended Use

The Cripto-1 ELISA is an enzyme immunoassay for measurement of Cripto-1 in human serum, plasma or urine.

Contents of Kit

1. Microtiter wells, 12 x 8 (break apart) strips, 96 wells; Wells coated with anti-Cripto-1 antibody (monoclonal).
2. Standard (Standard 0-5), 6 vials, 1 mL each, ready to use; Concentrations: 0; 50; 100; 200; 400; 800 pg/mL. Contain non-mercury preservative.
3. Control Low and High, 2 vials, 1 mL each, ready to use; For control values and ranges please refer to vial label or QC-Datasheet. Contain non-mercury preservative.
4. Assay Buffer, 1 vial, 7 mL, ready to use, Contains non-mercury preservative.
5. Enzyme Conjugate, 1 vial, 3 mL, ready to use, biotinylated mouse anti-Cripto-1 antibody; Contains non-mercury preservative.
6. Enzyme Complex, 1 vial, 14 mL, ready to use, Streptavidin-HRP conjugate. Contains non-mercury preservative.
7. Substrate Solution, 1 vial, 14 mL, ready to use, Tetramethylbenzidine (TMB).
8. Stop Solution, 1 vial, 14 mL, ready to use, contains 0.5M H2SO4.
9. Wash Solution, 1 vial, 30 mL (40X concentrated)

Storage

Store all contents at 2 to 8°C.

Detection Range

50-800 pg/mL

Sensitivity

8.4 pg/mL

General Description

Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene.