Human Connective Tissue Growth Factor, CTGF ELISA Kit (CKERS-CTGF-007H)

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Size
10 plates
Species Reactivity
Human
Intended Use
Human CTGF ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant CTGF in a sandwich ELISA format within the range of 63-4,000 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay CTGF in approximately 1,000 ELISA plate wells.
Contents of Kit
1. Capture Antibody: 100 μg of antigen-affinity purified goat anti-CTGF.
2. Detection Antibody: 50 μg of biotinylated antigen-affinity purified goat anti-CTGF.
3. Human CTGF Standard: 1 μg of recombinant CTGF.
Storage
Store the kit at 4°C upon receipt. For more detailed information, please download the following document on our website.
General Description
CTGF (connective tissue growth factor) is a cysteine-rich, matrix-associated, heparin-binding protein and a member of the CCN family of secreted cysteine rich regulatory proteins and is the major mitogenic and chemoattractant protein produced by umbilical vein and vascular endothelial cells. CTGF stimulates the proliferation and differentiation of chondrocytes, induces angiogenesis, promotes cell adhesion of fibroblasts, endothelial, and epithelial cells, and binds to IGF, TGF beta1, and BMP-4.
Reconstitution And Storage
Capture Antibody: 100μg of antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 100μg/ml. Following reconstitution the Capture antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
Detection Antibody: 50μg of biotinylated antigen-affinity purified goat anti-CTGF. Centrifuge vial prior to opening. Reconstitute in 0.5 ml sterile water for a concentration of 100μg/ml. Following reconstitution the Detection antibodies may be stored at 2-8°C for up to 6 months. For long term storage it is recommended to aliquot into working volumes and store at -70°C in a manual defrost freezer.
Human CTGF Standard: 1 μg of recombinant CTGF. Centrifuge vial prior to opening. Reconstitute in 1.0 ml sterile water for a concentration of 1 μg/ml. The Standard may be stored at 2-8°C for one month or aliquoted and stored at -70°C for up to three months in a manual defrost freezer.
UltraAvidin-HRP Conjugate: 40μl vial. Upon receipt, UltraAvidin-HRP conjugate should be stored at 2-8°C, DO NOT FREEZE.
TMB Liquid Substrate: Aspirate and wash plate 4 times. Add 100μl of TMB HRPO Microwell Substrate Standard Kinetic One Component "Ready Use" to each well. Incubate at room temperature and monitorcolor development with an ELISA plate reader at 450 nm with wavelength correction set at 540 nm or 570 nm. The reaction may be stopped after 15-20 minutes by adding 100μl of 2 M sulfuric acid to each well.

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