Intended Use
The HSP27 (Phospho-Ser78) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HSP27 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated HSP27 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HSP27 phosphorylation
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 Plates
2. 10x TBS: 24 mL (10x), Clear
3. Quenching Buffer: 24 mL (1x), Clear
4. Blocking Buffer: 50 mL (1x), Clear
5. 10x Wash Buffer: 50 mL (10x), Clear
6. 100x Anti-HSP27 (Phospho-Ser78) Antibody (Rabbit Polyclonal): 60 μL (100x), Red
7. 100x Anti-HSP27 Antibody (Rabbit Polyclonal): 60 μL (100x), Purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL (100x), Green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL (1x), Glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL (1x), Glass
11. Primary Antibody Diluent: 12 mL (1x), Clear
12. Ready-to-Use Substrate: 12 mL (1x), Brown
13. Stop Solution: 12 mL (1x), Clear
14. Crystal Violet Solution: 12 mL (1x), Glass
15. SDS Solution: 24 mL (1x), Clear
16. Adhesive Plate Seals: 4 Seals
Citations
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Almeida-Souza, L; Asselbergh, B; et al. MUTATIONS IN HSPB1 CAUSING PERIPHERAL NEUROPATHY STABILIZE MICROTUBULES. JOURNAL OF THE PERIPHERAL NERVOUS SYSTEM 18:5-6(2013).
Kondo, A; Tokuda, H; et al. Unphosphorylated heat shock protein 27 suppresses fibroblast growth factor-2-stimulated vascular endothelial growth factor release in osteoblasts. MOLECULAR MEDICINE REPORTS 8:691-695(2013).
COA
Certificate of Analysis
