Anti-Human HSPB1 Polyclonal Antibody

Detection of human HSP27 by western blot of immunoprecipitates.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.
Antibodies: Affinity purified antibody used for IP at 6 µg per reaction.
Detection Chemiluminescence with an exposure time of 30 seconds.

Detection of human HSP27 by immunohistochemistry.
Sample: FFPE section of human ovarian carcinoma.
Antibody: Antibody used at a dilution of 1:5,000 (0.2µg/ml).
Detection: DAB

Detection of human HSP27 by western blot.
Samples: Whole cell lysate (50 µg) from HeLa and 293T cells prepared using NETN lysis buffer.
Antibodies: Affinity purified antibody used for WB at 0.4 µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.