Species Reactivity
Human, Mouse, Rat
Detection Method
Indirect ELISA
Intended Use
The HDAC1 (Phospho-Ser421) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HDAC1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated HDAC1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HDAC1 phosphorylation.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate: 2 plates
2. 10x TBS: 24 mL
3. Quenching Buffer: 24 mL
4. Blocking Buffer: 50 mL
5. 10x Wash Buffer: 50 mL
6. 100x Anti-HDAC1 (Phospho-Ser421) Antibody (Rabbit Polyclonal): 60 μL, red
7. 100x Anti-HDAC1 Antibody (Rabbit Polyclonal): 60 μL, purple
8. 100x Anti-GAPDH Antibody (Mouse Monoclonal): 60 μL, green
9. HRP-Conjugated Anti-Rabbit IgG Antibody: 12 mL, glass
10. HRP-Conjugated Anti-Mouse IgG Antibody: 12 mL, glass
11. Primary Antibody Diluent: 12 mL
12. Ready-to-Use Substrate: 12 mL
13. Stop Solution: 12 mL
14. Crystal Violet Solution: 12 mL
15. SDS Solution: 24 mL
16. Adhesive Plate Seals: 4 seals
Citations
Have you cited DEIA-XYA773 in a publication?
Let us know and earn a reward for your research.
| Product Name |
Cat. No. |
Applications |
Host Species |
Datasheet |
Price |
Add to Basket |
| Product Name |
Cat. No. |
Applications |
Host Species |
Datasheet |
Price |
Add to Basket |
Wang, Q; Yin, S; et al. Histone deacetylation is required for orderly meiosis. CELL CYCLE 5:766-774(2006).
Karwowska-Desaulniers, P; Ketko, A; et al. Histone deacetylase 1 phosphorylation at S421 and S423 is constitutive in vivo, but dispensable in vitro. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 361:349-355(2007).
COA
Certificate of Analysis
