Product Overview
HeLa cell lines were engineered into double-knockout lines by CRISPR technology. The double knockout genotype was verified by PCR followed by sequencing. The HDAC1 knockout cell lysate are the cell homogenate in RIPA buffer made from the KO cell lines. A vial of lysate from the parental cell line was also provided as an internal control.
Alternative Names
HDAC1; histone deacetylase 1; HD1; RPD3; GON-10; RPD3L1; reduced potassium dependency, yeast homolog-like 1;
Application Notes
Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Dilution
Lysate samples can be diluted with 2x SDS Sample Buffer.
After dilution, the protein sample should be aliquoted and stored at -20°C for long term storage.
Concentration
The protein concentration was determined with BCA assay.
Storage
Store at -20°C. Avoid repeated freeze-thaw cycles.
Citations
Have you cited DAG-KO114 in a publication?
Let us know and earn a reward for your research.
| Product Name |
Cat. No. |
Applications |
Host Species |
Datasheet |
Price |
Add to Basket |
| Product Name |
Cat. No. |
Applications |
Host Species |
Datasheet |
Price |
Add to Basket |
Underhill, C; Qutob, MS; et al. A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1. JOURNAL OF BIOLOGICAL CHEMISTRY 275:40463-40470(2000).
Ryu, JK; Kim, WJ; et al. Inhibition of histone deacetylase 2 mitigates profibrotic TGF-beta 1 responses in fibroblasts derived from Peyronie's plaque. ASIAN JOURNAL OF ANDROLOGY 15:640-645(2013).
COA
Certificate of Analysis
