Human I-FABP ELISA kit

Sample

serum, plasma, urine and cell culture supernatant samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The human I-FABP ELISA kit is to be used for the in vitro quantitative determination of human I-FABP in serum, plasma, urine and cell culture supernatant samples. This kit is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures.
The analysis should be performed by trained laboratory professionals.

Storage

1. Upon receipt, store individual components at 2 - 8°C. Do not freeze.
2. Do not use components beyond the expiration date printed on the kit label.
3. The standard and tracer in lyophilized form and the streptavidin-peroxidase in concentrated solution are stable until the expiration date indicated on the kit label, if stored at 2 - 8°C.
4. The exact amount of the standard is indicated on the label of the vial and the Certificate of Analysis.
5. Once reconstituted the tracer is stable for 1 month if stored at 2-8°C.
6. The streptavidin-peroxidase can only be stored in concentrated solution and is not stable when stored diluted.
7. Upon receipt, foil pouch around the plate should be vacuum-sealed and unpunctured. Any irregularities to aforementioned conditions may influence plate performance in the assay.
8. Return unused strips immediately to the foil pouch containing the desiccant pack and reseal along the entire edge of the zip-seal. Quality guaranteed for 1 month if stored at 2 - 8°C.

Precision

The intra-assay precision and reproducibility was determined with four plasma samples that were tested in duplicate.

To determine the inter-assay variation, four identical plasma samples were tested on two plates by two different operators.

To determine batch-to-batch variation, four different internally validated plasma samples have been applied in plates of five different batches and used as an internal control.

General Description

Fatty acid-binding proteins (FABPs) are a class of cytoplasmic proteins that bind long chain fatty acids. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. Intestinal FABP (I-FABP) is specifically localized in the epithelium cells of the small bowel.
The I-FABP protein is derived from the human FABP2 gene. Normally, I-FABP is undetectable in serum. Many observations indicate that I-FABP is a useful biochemical marker for intestinal cell damage both in vivo and in vitro. Ischemically damaged cells are characterized histologically by absence (or low presence) of FABP facilitating recognition of areas of ischemically damaged cells.