Sample
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Intended Use
For the quantitation of Human FABP2 concentrations in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Storage
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Precision
cd spends great efforts in documenting lot-to-lot variability and ensuring our assay kits produce robust data that are reproducible.
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Detection Range
31.2 pg/ml - 2,000 pg/ml
General Description
Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.
Standard Curve
The highest O.D. value might be higher or lower than in the example. The experiment result is statistically significant if the highest O.D. value is no less than 1.0.
A standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Citations
Publication ()
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COA
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