Estrone [HRP]

Name: Estrone [HRP]
SKU: DAGB348
Rating: 5 (1 reviews)

Estrone, HRP-conjugated, Synthetic antigen

COA

SDS

Datasheet

Summary

Specificity

Each conjugate comprises antigen covalently bound to horseradish peroxide and is suitable as a tracer in immunoassay development.

Nature

Synthetic

Tag/Conjugate

HRP

Alternative Names

estrone

Format

The conjugate is supplied as a concentrate. Dilute as required and use working strength conjugate immediately after dilution

Preservative

None

Storage

2 - 8°C for up to 3 months / -20°C for long term storage

Introduction

Estrone is one of the three naturally occurring estrogens, the others being estradiol and estriol. Estrone is produced primarily from androstenedione originating from the gonads or the adrenal cortex. In premenopausal women, more than 50% of the estrone is secreted by the ovaries. In prepubertal children, men and non supplemented postmenopausal women the major portion of estrone is derived from peripheral tissue conversion of androstenedione. Interconversion of estrone and estradiol also occurs in peripheral tissue. Bioassay data indicate that the estrogenic action is much less than estradiol. Estrone is a primary estrogenic component of several pharmaceutical preparations, including those containing conjugated and esterified estrogens. In premenopausal women estrone levels generally parallel those of estradiol. After menopause estrone levels increase, possibly due to increased conversion of androstenedione to estrone.
References

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Enhanced estrogen removal in activated sludge processes through the optimization of the hydraulic flow pattern

WATER RESEARCH

Authors: Coello-Garcia, T.; Curtis, T. P.; Mrozik, W.; Davenport, R. J.

Abstract

The removal of fi-estradiol (E2) and alpha-ethinylestradiol (EE2) in biological wastewater treatment plants (WWTP) would need to be improved in order to comply with prospective Environmental Quality Standards (EQS) of 0.4 and 0.035 ng.L-1 respectively. The effluent concentration of a micropollutant in an activated sludge process is a function of the removal rate, the hydraulic retention time (HRT) and the flow pattern, which is usually overlooked. In order to better understand this aspect, we carried out tracer studies in eight WWTPs in the UK and found that relatively modest changes in aeration tanks would translate into tangible improvements in their flow pattern. We further evaluated the degradation rates for El (estrone), E2, E3 (estriol) and EE2 in each WWTP and we estimated that the modification of the flow pattern would be sufficient to place effluent concentrations of E2 (23.2 L.giA " d(-1)

Liquid chromatography tandem mass spectrometry determination of 34 priority and emerging pollutants in water from the influent and effluent of a drinking water treatment plant

JOURNAL OF CHROMATOGRAPHY A

Authors: Borrull, Josep; Colom, Agusti; Fabregas, Josepa; Borrull, Francesc; Pocurull, Eva

Abstract

This study evaluates the applicability of a method based on the direct injection of a large volume of water samples to identify and quantify 34 priority and emerging substances, most of them discussed in Directive 2013/39/EU on priority substances in the field of water policy, and Decision 2018/840/EU (Watch List). The method directly injects 500 mu L of filtered water sample and so does not use a pre-concentration step. The method was satisfactorily validated for influent and effluent water from a drinking water treatment plant, at three concentrations (1, 10 and 100 ng L-1) with precision and accuracies in the range 1-17% and 71-122% respectively. Sensitivity was good with detection limits in the range 0.15-10 ng L-1 and complied with EU limits in all cases except for estrone, 17-beta-estradiol and 17-alpha-ethinylestradiol. For these hormones, an on-line solid phase extraction was developed and evaluated. The methods were applied to the analysis of water collected at the influent and effluent of a drinking water treatment plant and revealed the presence of 18 of the target compounds in the influent water and 8 in the effluent water. This showed that most the compounds had been efficiently removed by the processes of the drinking water treatment plant. (C) 2020 Elsevier B.V. All rights reserved.